子宫颈癌患者可溶性尿激酶受体、鳞状细胞癌抗原、人乳头瘤病毒16、人乳头瘤病毒18联合检测的意义  

Significance of detection of suPAR, SCC-Ag and HPV16, 18 in patients with cervical cancer

作　　者：郭勇峰[1] 郑曙民[1] 李新燕[2] 

机构地区：[1]山西省肿瘤医院妇一科,太原030013 [2]山西省妇幼保健院妇产科

出　　处：《肿瘤研究与临床》2014年第12期837-839,共3页Cancer Research and Clinic

摘　　要：目的 探讨可溶性尿激酶受体（suPAR）、鳞状细胞癌抗原（SCC-Ag）、人乳头瘤病毒（HPV） 16、HPV18联合检测在子宫颈癌患者病情监测及预后评估中的意义.方法 选择206例子宫颈癌患者及60名健康对照者血液和子宫颈分泌物标本,通过酶联免疫吸附试验（ELISA）检测血浆suPAR及SCC-Ag水平,用荧光定量反转录聚合酶链反应法检测子宫颈分泌物HPV16、HPV18的表达,并分析三者的相关性.结果 子宫颈浸润癌患者血浆suPAR、SCC-Ag水平均比健康对照组升高[（1.072 5±0.305 2）ng/ml比（0.501 7 ±0.179 3）ng/ml、（0.980 6±0.162 7）μg/ml比（0.261 4±0.006 3）μg/ml],差异有统计学意义（均P＜ 0.05）;子宫颈浸润癌患者及原位癌患者分泌物HPV16、HPV18阳性表达率分别为53.89％（90/167）和46.15％（18/39）,比健康对照组（6.67％,4/60）升高,差异有统计学意义（P＜0.05）.子宫颈浸润癌患者血浆suPAR水平与SCC-Ag水平呈正相关（r=0.564,P＜0.05）,子宫颈浸润癌患者子宫颈分泌物HPV16、HPV18表达阳性组和阴性组的血浆suPAR水平差异无统计学意义（P＞0.05）.结论 子宫颈浸润癌患者血浆suPAR水平与SCC-Ag水平存在正相关关系,尚不能认为子宫颈浸润癌患者血浆suPAR水平与感染HPV16、HPV18有关.Objective To evaluate the significance of suPAR,SCC-Ag in plasma and HPV16,18 in cervical secretion for monitoring pathogenetic condition and prognosis in patients with cervical cancer.Methods 206 cervical cancer patients＆quot; blood and cervical secretion were collected.Plasma level of suPAR and SCC-Ag were measured by enzyme linked immunosorbent assay （ELISA） in health women and patients with cervical cancer.The expression of HPV16,18 of cervical secretion in control group and patients with cervical cancer were detected by fluorescence quantitative PCR.The correlations of the three indexes were analyzed.Results The plasma level of suPAR and SCC-Ag,the expression of HPV16,18 of cervical secretion in cervical cancer patients were obviously higher than those in health controls with statistical significance （（1.072 5±0.305 2） ng/ml vs （0.501 7±0.179 3） ng/ml,（0.980 6±0.162 7） μg/ml vs （0.261 4± 0.006 3） μg/ml and 53.89 % （90/167）,46.15 % （18/39） vs 6.67 % （4/60）,P 〈 0.05）.There was a positive correlation between plasma suPAR level and SCC-Ag level in invasive carcinoma of cervix patients （r =0.564,P 〈 0.05）.The plasma level of suPAR between in HPV16,18 positive group and in HPV16,18 negative group did not show difference （P 〉 0.05）.Conclusions In invasive carcinoma of cervix patients,there is a positive correlation between plasma suPAR level and SCC-Ag level.But it＆#39;s not yet to conclude that plasma suPAR level of cervix invasive carcinoma patients is related to infection of HPV16,18.

关 键 词：子宫颈肿瘤 可溶性尿激酶受体 鳞状细胞癌抗原 人乳头瘤病毒16 人乳头 瘤病毒18 

分 类 号：R737.33[医药卫生—肿瘤]