利用SPME-GC×GC/TOFMS对香草兰中挥发、半挥发性成分萃取与分析研究  

Analysis of Volatile and Semi-volatile Compounds in Vanilla Using Solid Phase Micro-Extraction-Comprehensive Two Dimensional Gas Chromatography/Time of Flight Mass Spectrometry

作　　者：谷风林[1,2] 董智哲 徐飞[1,2] 赵建平[1,2] 

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]农业部香辛饮料作物遗传资源利用重点实验室,海南万宁571533 [3]华中农业大学食品科技学院,湖北武汉430070

出　　处：《热带作物学报》2015年第1期185-191,共7页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划课题(No.2012BAD36B03);中央级公益性科研院所基本科研业务费专项(No.1630052012017)

摘　　要：利用固相微萃取(SPME)法萃取香草兰中挥发和半挥发性的成分,再结合全二维气相色谱/飞行时间质谱(GC×GC/TOFMS)技术进行定性分析。试验共鉴定出香草兰中181种挥发、半挥发性化合物,其中含量较高的成分主要有香兰素、呋喃甲醛、(2S,3S)-(+)-2,3-丁二醇、邻甲氧基苯酚、2,3-丁二醇、苯酚、正己醛、(2R,3R)-(-)-2,3-丁二醇、对羟基苯甲醛、3-甲基丁酸、2,3,5,6-四甲基吡嗪、3-羟基-2-丁酮、香草酸、壬醛、正己酸、1-辛烯-3-酮。结果表明,GC×GC/TOFMS高分辨率和高灵敏度的特点非常适合复杂组分体系的定性分析。Volatile and semi-volatile compounds in vanilla were extracted by using solid phase micro-extraction and were identified by comprehensive two-dimensional gas chromatography/time of flight mass spectrometer. A total of 181 volatile or semi-volatile compounds in vanilla were identified and compounds with high content were vanillin, 2-furancarboxaldehyde, [S- （R＊,R＊）] -2,3-butanediol, 2-methoxy-phenol, 2,3-butanediol, phenol, hexanal, [R- （R＊,R＊）] -2,3-butanediol, 4-hydroxy-benzaldehyde, 3-methyl-butanoic acid, tetramethyl-pyrazine, 3-hydroxy- 2-butanone, 4-hydroxy-3-methoxy-benzoie acid, nonanal, hexanoic acid, 1-octen-3-one.The results showed that GCxGC/TOFMS with high resolution and sensitivity is suitable for the identification of complex system.

关 键 词：香草兰 全二维气相色谱 飞行时间质谱 

分 类 号：TQ651[化学工程—精细化工]