急性胰腺炎中E-钙粘蛋白的诊断价值  

The diagnosis value of E-cadherin in acute pancreatitis of rat

作　　者：袁伟红[1] 潘琦[1] 陈国昌[1] 陈义钢[2] 

机构地区：[1]宜兴市人民医院消化科,江苏宜兴214200 [2]上海交通大学医学院,200025

出　　处：《重庆医学》2015年第2期168-170,共3页Chongqing medicine

摘　　要：目的探讨E-钙粘蛋白(E-cad)在急性胰腺炎中的表达及对其严重性的诊断价值。方法 24只雄性SD大鼠随机均分为对照组、轻症急性胰腺炎组(MAP组)和重症急性胰腺炎组(SAP组)。通过腹腔注射L-精氨酸造模,建模24h后测定血清中淀粉酶活性及E-cad表达变化,胰腺组织进行病理组织学观察,蛋白免疫印迹(Western blot)检测胰腺组织E-cad的蛋白表达。结果 MAP组和SAP组胰腺病理评分、血清淀粉酶值明显高于对照组(P<0.05),SAP组与MAP组比较差异也有统计学意义(P<0.05);血清E-cad酶联免疫吸附试验(ELISA)检测结果显示:MAP组(1 025.50±131.33)pg/mL和SAP组(1 561.75±144.82)pg/mL均明显高于对照组(626.50±72.12)pg/mL,且两组之间比较差异也有统计学意义(P<0.05);Western blot检测胰腺组织中SAP组E-cad表达强于对照组及MAP组。结论 E-cad在急性胰腺炎早期表达明显增高,对其严重性具有一定的诊断价值。Objective To explore the diagnosis value and expression of E‐cadherin in the acute pancreatitis .Methods Twenth‐four male SD rats were randomly divided into control group ,mild acute pancreatitis （MAP） group and severe acute pancreatitis （SAP） group ,8 case in each groups .The animal model was established by intraperitoneal injection of L‐arginine .After 24 hours ,the serum levels of amylase and the expression change of variation E‐cadherin were tested .The pancreas tissue was taken for his‐topathological score under microscope .The expression of E‐cadherin at protein level in pancreatic tissue was detected by Western Blot .Results The histological scores of pancreas and serum amylase were significantly higher in the MAP and SAP groups than those in control group （P〈0 .05） ,and difference between SAP group and MAP group was also very significant（P〈0 .05） .The re‐sult of ELISA showed that the serum E‐cadherin in MAP group[（1 025 .50 ± 131 .33） pg/mL] and SAP group[（1 561 .75 ± 144 .82）pg/mL] were significantly higher than that in control group[（626 .50 ± 72 .12） pg/mL ,P〈0 .05] ,and there was significant difference between MAP group and SAP group（P〈0 .05） .The result of Western Blot was similar to the result of ELISA .Conclu‐sion E‐cadherin may have diagnosis value in the acute pancreatitis .

关 键 词：胰腺炎 诊断 E-钙粘蛋白 

分 类 号：R576[医药卫生—消化系统]