半乳糖凝集素3与Bcl-2在溃疡性结肠炎患者大肠黏膜中的应用研究  

Application research of Galectin-3 and Bcl-2 in colorectal tissues of patients with ulcerative colitis

作　　者：张启芳[1] 李晓燕[2] 王媛媛[3] 李西融[3] 陈卓琳[2] 郑奕[2] 何思明[2] 陈勇昌[2] 姜海行[1] 

机构地区：[1]广西医科大学第一附属医院消化内科,南宁530021 [2]广西壮族自治区南溪山医院消化内科,广西桂林541002 [3]广西壮族自治区南溪山医院病理科,广西桂林541002

出　　处：《重庆医学》2015年第2期180-182,185,共4页Chongqing medicine

基　　金：广西壮族自治区卫生厅科研项目(Z2011025)

摘　　要：目的探讨溃疡性结肠炎(UC)患者大肠黏膜组织中凋亡蛋白半乳糖凝集素3(Gal-3)与B淋巴细胞瘤-2基因(Bcl-2)的表达及意义。方法应用免疫组织化学SP法检测UC患者60例(UC组)和正常对照者20例(对照组)大肠黏膜组织中的Gal-3与Bcl-2的表达,并分析UC大肠黏膜组织中Gal-3与Bcl-2表达的关系。以细胞质或细胞核中出现明显的棕黄色颗粒为阳性细胞,根据染色强度及阳性细胞数进行光镜下半定量分析。结果 Gal-3与Bcl-2的表达均在上皮细胞的细胞质,Gal-3在正常大肠黏膜上皮细胞中呈强阳性表达,在UC大肠上皮细胞的表达明显减弱,且与肠镜下病变程度相关;Bcl-2在正常大肠黏膜上皮细胞中呈弱表达,在UC大肠黏膜上皮细胞的表达明显增强,主要为固有层炎性细胞表达增强,与肠镜下病变程度无明显关系。Gal-3与Bcl-2的表达与患者的年龄、性别、病程均无明显相关。Pearson相关分析显示Gal-3与Bcl-2的阳性表达程度不具有关联性。结论 Gal-3与Bcl-2蛋白在UC的发生和发展中起重要作用,可望成为UC早期诊断和预后判断的指标,两者在UC的发病中可能独立起作用。Objective To explore the expressions and significance of Galectin‐3 and Bcl‐2 in colorectal tissues of patients with ulcerative colitis（UC） .Methods Immunohistochemical SP method was applied to detected the expression of Galectin‐3 and Bcl‐2 in colorectal tissues of 60 patients in UC group and 20 healthy adults in the control group ,and analyzed the relationship of the expres‐sions between Galectin‐3 and Bcl‐2 .It was regarded as positive cell when obvious dark brown granules appeared in cytoplasm or cyteblast .Semi‐quantitative analysis was used basing on the staining intensity and the amount of the staining intensity and positive cells .Results Galectin‐3 and Bcl‐2 proteins expressed in cytoplasm .Galectin‐3 showed strong expression in normal colorectal epi‐thelium but weak in UC inflammatory tissues ,and it was associated with different lesion degrees under endoscopy .The expressions of Bcl‐2 were weak in normal colorectal epithelium ,and it enhanced significantly in UC inflammatory tissues ,especially in inflamma‐tory cells of laminae propria ,and it was not associated with different lesion degrees under endoscopy .The expression of Galectin‐3 and Bcl‐2 was not associated with the age ,sex of patients and the course of UC .Pearson correlation analysis showed that the posi‐tive expressions of Galectin‐3 and Bcl‐2 had no relevance .Conclusion Galectin‐3 and Bcl‐2 involved in the pathogenesis of UC . They may be able to used as markers of early diagnosis and prognosis in UC and may play the role in the pathogenesis of UC inde‐pendently .

关 键 词：结肠炎  溃疡性 半乳糖凝集素3 免疫组织化学 B淋巴细胞瘤-2基因 

分 类 号：R574.1[医药卫生—消化系统]