茶树Bt和Cpti双价抗虫基因转化研究(简报)  

Transform of Bt and Cpti into tea plant(Camellia sinesis)

作　　者：成杨[1] 赵洋[1] 杨培迪[1] 杨阳[1] 刘振[1] 

机构地区：[1]湖南省茶叶研究所,湖南长沙410125

出　　处：《茶叶通讯》2014年第4期41-43,共3页Journal of Tea Communication

基　　金：现代农业产业技术体系(CARS-23)

摘　　要：利用转基因获得抗虫茶树品种是防治茶树害虫的重要途径。将含有Bt和Cpti双价抗虫基因的质粒转入感受态E.coitl DH5α菌株并进行培养、提取和扩增后的质粒,注入茶树花期的子房内,种植接种后的茶籽,并对获得的植株进行检测。To obtain pest resistence by genetically modification is one of the important techinique in tea pest control. Plasmid with Bt and Cpti was introduced into competent E.coli DH5a strain, and cultured. The amplified plasmid was extracted and injected into florescene tea ovary. The inoculated tea seed was planted, and the growing up tea tree was tested.

关 键 词：茶树 花粉管通道法 BT CPTI 

分 类 号：S571.1[农业科学—茶叶生产加工]