杉木炭疽病拮抗菌HY32的发酵条件优化  

Optimization of Fermentation Medium Formula and Culture Conditions for the Antagonistic Bacterium HY32 against Colletotrichum gloeosporioides

作　　者：李红军[1] 周国英[1] 路宗岩[1] 谭益民[1] 段爱国[2] 

机构地区：[1]中南林业科技大学林学院,长沙410004 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学》2014年第12期94-100,共7页Scientia Silvae Sinicae

基　　金：国家林业公益性行业科研专项(201001014)

摘　　要：采用单因素试验法和响应面法,探讨杉木炭疽病拮抗菌HY32发酵培养基及发酵条件的优化途径。结果表明:该菌株最佳发酵培养基的最适碳源为葡萄糖,最适氮源为酵母膏和蛋白胨;最佳培养基配方为葡萄糖19.80 g·L-1、酵母膏12.16 g·L-1、蛋白胨4.22 g·L-1、Na Cl13.13 g·L-1。该菌株最佳发酵培养条件为培养温度30.52℃、初始p H8.09、摇床转速184.67 r·min-1。验证结果表明采用响应面法优化菌株HY32的发酵培养基及发酵条件是可行的。Bacterium HY32 was identified as an antagonistic strain against Colletotrichum gloeosporioides previously. Both the fermentation medium formula and culture conditions for the antagonistic bacterium HY32 were optimized by using a single factor test and a response surface methodology （RSM） in this study. The test results indicated that the optimized carbon source was glucose, nitrogen source was yeast extract and peptone, and inorganic salt was NaC1. Based on the single facfor test, the optimized concentration of these four major media components was determined. The OD60o （optical density at 600 nm） value of cultures after 48 h fermentation was measured to assess the fermentation rate. The analyzed data indicated that the optimal medium was as the following： glucose 19.80 g·L^-1, yeast extract 12. 16 g·L^-1, peptone 4.22 g·L^-1 and NaC1 13.13 g·L^-1. With this optimal medium, we found that the actual OD600 value of HY32 culture was able to reach a high level of 1. 743 after 48 h fermentation. Single factor test results also showed that the initial pH value, culture temperature and rotation speed were key factors to influence the bacterial growth and inhibitory rate. The best culture conditions were determined as the following： culture temperature 30.52 ℃ , pH 8.09 and rotation speed 184.67 r· min^ - 1 respectively.

关 键 词：杉木 拮抗菌HY32 发酵条件 响应面法 

分 类 号：S763.15[农业科学—森林保护学]