脐橙果肉中噻菌灵农药的SERS快速检测研究  

Rapid Detection of Thiabendazole Residues in Navel Orange Flesh by SERS

作　　者：李俊杰[1] 严霖元[1] 刘木华[1] 骆鹏杰 吴瑞梅[1] 王晓彬[1] 陈金印[3] 

机构地区：[1]江西农业大学工学院,江西南昌330045 [2]国家食品安全风险评估中心,北京100022 [3]江西农业大学农学院,江西南昌330045

出　　处：《江西农业大学学报》2014年第6期1229-1233,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省学术带头人培养计划(20113BCB22001);赣鄱英才555工程人选计划;江西省科技攻关项目(20112BDH80010)

摘　　要：采用表面增强拉曼光谱技术快速分析脐橙果肉中的噻菌灵农药残留。以液质联用方法未检测出含噻菌灵农药的脐橙为对象，利用乙腈快速提取脐橙果肉汁，分别采集不同浓度噻菌灵标准溶液和以脐橙果肉提取液为基质的噻菌灵溶液的表面增强拉曼信号。结果表明，噻菌灵的拉曼特征频率较为丰富，选取1010 cm－1处的特征峰制定噻菌灵的标准曲线，其特征峰强度与噻菌灵标准溶液的浓度（1～50 mg／L）呈良好的线性关系。对以脐橙果肉提取液为基质的不同浓度噻菌灵溶液的SERS分析表明，利用该方法快速检测脐橙果肉中噻菌灵溶液的最低检测浓度为5 mg／kg，该浓度下的784，1009，1276 cm－1处的特征峰依然明显，与噻菌灵标准溶液的特征峰振动归属一致，可以作为判别脐橙果肉中噻菌灵农药残留的依据。This paper investigates the feasibility of detecting rapidly the thiabendazole residues in navel orange flesh by Surface-enhanced Raman Spectroscopy （SERS）.Acetonitrile was used to rapidly extract navel orange flesh juices,from which no thiabendazole was detected by LC-MS method.SERS of different concentrations of thiabendazole standard solutions and thiabendazole solutions with orange flesh extraction as the matrix were collected by laser Raman spectrometer.The results showed that thiabendazole had rich Raman characteristic frequencies.The standard curve was plotted with the intensity of the peak 1 010 cm-1 and the SERS signals of thiabendazole standard solutions （1~50 mgL） had a good linear relationship.Thiabendazole solutions with orange flesh extract as the matrix can be detected by SERS method in low concentrations of 5 mgkg and the characteristic peaks of 784,1 009 and 1 276 cm-1were still obvious.The vibrational modes of these characteristic peaks were consistent with thiabendazole standard solutions.They can be regarded as the discriminative basis of thiabendazole pesticide.

关 键 词：表面增强拉曼光谱 噻菌灵 脐橙果肉 快速检测 

分 类 号：TS207.53[轻工技术与工程—食品科学]