碱性成纤维细胞生长因子对染铅大鼠海马神经元胞浆ERK的保护作用  

Protection of bFGF on ERK in Cytosol of Hippocampal Neuron Exposed to Lead

作　　者：吴攀峰[1] 焦铭[2] 孙慧荣[3] 吴基良[1,2] 

机构地区：[1]武汉大学基础医学院药理系,湖北武汉430071 [2]湖北科技学院糖尿病心脑血管病变湖北省重点实验室 [3]武汉大学附属中南医院心内科

出　　处：《湖北科技学院学报（医学版）》2014年第6期461-462,465,共3页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院糖尿病专项基金项目(ZX1006)

摘　　要：目的研究碱性成纤维细胞生长因子（b FGF）对染铅大鼠海马神经元细胞外调节蛋白激酶（ERK）的保护作用。方法神经元培养7 d后,用0.6μg/L的b FGF以及不同浓度铅处理细胞,然后测定ERK活力。结果铅处理神经元30 min后,在铅浓度0.1~2.0μmol/L时ERK活性明显升高,其中2.0μmol/L时最高。经过b FGF处理以后的细胞,在铅浓度1.0~4.0μmol/L时ERK活性与对照组相比无明显差异。结论染铅浓度不同对ERK活性影响也不同,b FGF可以保护神经元中ERK活性。Objective To study the protection of basic fibroblast growth factor（ b FGF） on the activity of extracellular signal-regulated kinase（ ERK） in hippocampal neuron of rats exposed to lead. Methods After cultured for 7 days,the hippocampal neuron was treated with 0. 6 μg / L b FGF and lead with different concentration. The activity of ERK was then determined. Results The activity of ERK was increased when treated with0. 1 ~ 2. 0 μmol / L lead for 30 min. While treated with b FGF in advance,the activity of ERK had no significant difference compared with the control group. Conclusion The activity of ERK was changed by treated with different concentrations of lead,and b FGF can protect ERK activity in neuron.

关 键 词：碱性成纤维细胞生长因子 铅 海马神经元 细胞外调节蛋白激酶 

分 类 号：R741.02[医药卫生—神经病学与精神病学]