沉默Caveolin-1基因对膀胱癌细胞增殖和凋亡的影响  

Effect of the Silencing of Caveolin-1 Gene on Proliferation and Apoptosis of Human Bladder Cancer Cells

作　　者：袁渊[1] 罗婷婷[1] 姚启盛[1] 杨勇[1] 陈从波[1] 黄力[1] 龚小新[1] 

机构地区：[1]湖北医药学院附属太和医院泌尿外科,湖北十堰442000

出　　处：《医学临床研究》2014年第12期2358-2360,共3页Journal of Clinical Research

摘　　要：目的探讨沉默 Caveolin-1（Cav-1）基因对人膀胱癌细胞株5637增殖和凋亡的影响。方法通过siRNA转染人膀胱癌细胞株5637（沉默组）,以转染 FAM-siRNA为阴性对照组,未转染细胞为正常对照组。Re-al-time PCR法检测Cav-1基因沉默的效率,CCK-8法检测各组细胞的增殖情况,流式细胞术检测各种细胞的凋亡情况。结果 siRNA转染可显著降低人膀胱癌细胞株5637中 Cav-1 mRNA 的表达水平。沉默组细胞的增强能力明显减低,而细胞凋亡率明显升高（均P＜ 0.05）。结论沉默Cav-1基因可抑制人膀胱癌细胞株5637的细胞增殖并促进细胞凋亡。Objective]To explore the effect of the silencing of caveolin-1（Cav-1）gene on the proliferation and apoptosis of human bladder cancer cell line 5637 in vitro.[Methods]Cav-1 gene was transfected into human blad-der cancer cell line 5637 by siRNA（silence group）.Negative control group was transfected with FAM-siRNA, while normal control group was not transfected with FAM-siRNA.Real-time PCR was used to detect the efficacy of the silencing of CAV-1 gene.CCK-8 method was used to detect the cell proliferation in each group.Cell apopto-sis was measured by flow cytometry.[Results]SiRNA transfection could significantly reduce the mRNA expres-sion of Cav-1 in human bladder cancer cell line 5637.In the silence group,cell proliferation was obviously de-creased and cell apoptosis was obviously increased（all P〈 0.05）.[Conclusion]The silencing of Cav-1 gene can in-hibit the proliferation and promote the apoptosis of human bladder cancer cells.

关 键 词：细胞质膜微囊蛋白/遗传学 基因沉默 膀胱肿瘤/病理学 细胞增殖 细胞凋亡 

分 类 号：R737.14[医药卫生—肿瘤]