外源调控叶酸代谢对谷氨酸棒杆菌SYPS-062积累L-丝氨酸的影响  

Effect of exogenous regulation of folate metabolism on L-serine accumulation of Corynebacterium glutamicum SYPS-062

作　　者：徐国强[1,2] 袁圣男 任建洪[1] 张晓梅[1] 许正宏[1,2] 

机构地区：[1]江南大学药学院制药工程研究室,江苏无锡214122 [2]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122

出　　处：《食品与发酵工业》2014年第9期1-6,共6页Food and Fermentation Industries

基　　金：863项目(No.2012AA022102);广东省教育厅项目(No.2012B091000083)

摘　　要：通过外源添加叶酸合成前体对氨基苯甲酸、叶酸途径抑制剂氨甲喋呤扰动叶酸代谢,考察了叶酸对谷氨酸棒杆菌SYPS-062合成L-丝氨酸的影响。实验结果表明,添加对氨基苯甲酸（0-0.4μg/mL）能够促进菌体的生长（最大值为7.8±0.24 g/L）,但可显著降低其产酸能力;而添加氨甲喋呤（0-100μg/mL）抑制了谷氨酸棒杆菌SYPS-062的生长,但可提高其单位菌体产酸的能力;代谢通量分析表明,作为调控点的叶酸代谢在SYPS-062生长及积累L-丝氨酸中占主导地位。This study aimed to investigate the effects of exogenous regulation of cofactor folate on C. glutamicum SYPS-062 growth and L-serine accumulation by adding the precursor （p-aminobenzoate, PABA） or inhibitor （metho- trexate） of folate biosynthesis to the medium, respectively. The results indicated that the biomass enhanced and reached a maximum value of 7.8 - 0.24 g/L, while the concentration of L-serine sharply declined as the concentra- tion of PABA was increased from 0 to 0.4 μg/mL. In contrast, the biomass of C. glutamicum S-PS-062 decreased while the L-serine yield to biomass increased with the increase in the concentration of methotrexate from 0 to 100 μg/ mL. Metabolic flux analysis showed that folate metabolism played an important role in cell growth and L-serine accu- mulation of C. glutamicum SYPS-062.

关 键 词：谷氨酸棒杆菌 叶酸代谢 L-丝氨酸 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]