实时荧光聚合酶链式反应检测转基因小麦B73-6-1、B72-8-11b和B102-1-2品系  

A Real-Time Polymerase Chain Reaction Method to Detect Genetically Modified Wheat Strains B73-6-1, B72-8-11b and B102-1-2

作　　者：曹际娟[1] 徐君怡[1] 曹冬梅[1] 张丕桥[1,2] 栾凤侠 刘洋[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]辽宁师范大学生命科学学院,辽宁大连116029 [3]黑龙江出入境检验检疫局,黑龙江哈尔滨150001

出　　处：《食品科学》2014年第8期156-159,共4页Food Science

基　　金：国家转基因重大专项(2013ZX08012-001)

摘　　要：目的:建立实时荧光聚合酶链式反应检测转基因小麦B73-6-1、B72-8-11b和B102-1-2品系的鉴定方法。方法:以转基因小麦B73-6-1、B72-8-11b和B102-1-2为研究对象,在转基因小麦外源片段与小麦染色体重组的边界序列分别设计引物和探针,以其他多种转基因产品和非转基因小麦为对照进行特异性实验,以B73-6-1样品模拟制备10个含量梯度的添加样品进行灵敏度实验。结果:本研究设计的引物和探针具有很好的品系鉴定特异性,检测灵敏度可达到0.01%(m/m)。结论:该方法特异性好、灵敏度高,可快速、准确地鉴定转基因小麦B73-6-1、B72-8-11b和B102-1-2品系。Aim：To establish a real-time PCR method for the identification of transgenic wheat strains B73-6-1,B72-8-11b and B102-1-2.Methods：Specific primers and probes were designed according to the flanking sequences of exogenous fragments of transgenic wheat and wheat chromosomes.The specificity of the developed method was validated by using it to detect a variety of other genetically modified crops and non-genetically modified wheat strains.B73-6-1 was used to prepare 10 content gradients to test the sensitivity.Results：The primers and probes designed showed good detection specificity for transgenic wheat strains,with its sensitivity reaching 0.01％ （m/m）.Conclusion：A real-time PCR method for the identification of three genetically modified wheat strains has been established.This method is of high specificity,high reproducibility,rapid identification and excellent accuracy for the identification of genetically modified wheat strains B73-6-1,B72-8-11b and B102-1-2.

关 键 词：转基因小麦 B73-6-1 B72-8-11b B102-1-2 品系鉴定 实时荧光聚合酶链式反应 

分 类 号：Q812[生物学—生物工程]