酶连接探针杂交芯片特异性检测转基因水稻品系  

Event-specific Detection of Genetically Modified Rice Using Enzyme-Linked Probe Hybridization Chip

作　　者：张明哲 陈吴健 张晓峰 陈笑梅 陈曦 吴蓉 

机构地区：[1]浙江省检验检疫科学技术研究院,浙江杭州310016 [2]浙江出入境检验检疫局,浙江杭州310016 [3]杭州出入境检验检疫局,浙江杭州310012

出　　处：《食品科学》2014年第8期222-225,共4页Food Science

基　　金：浙江省科技厅重大科技专项重点农业项目(2011C12023);国家质检总局科技计划项目(2011IK249);浙江省科技厅公益性项目(2011C22065);浙江出入境检验检疫局科技计划项目(ZK200958)

摘　　要：酶连接探针杂交芯片是一种基于连接酶催化的探针杂交芯片技术,其原理是采用硫代引物保护法,利用Lambda DNA外切酶将多重聚合酶链式反应产物切割成单链。在氨基修饰的芯片上固定5’端探针,并与制备的产物单链进行杂交,加入荧光修饰的第2个探针与之前的探针进行酶连接反应,从而实现高特异性、高通量检测。结果表明:酶连接探针杂交芯片技术检测4种转基因水稻品系特异性好,灵敏度可达0.1%(m/m),可以应用于进出口检验检疫、食品安全监测等领域。The enzyme-linked probe hybridization chip is a method based on ligase-hybridizing probe chip technology with the principle of using thio-primers for protection,and using Lambda DNA exonuclease to cut multiple PCR amplicons into single products.5＇-End probe chain is fixed in the amino-modified chip,and hybridized with the single-chain product by adding the fluorescent-modified probe for the enzyme-linked reactions with the anterior probe in order to achieve highly specific,parallel and high-throughput detection.In this study,the enzyme-linked probe hybridization chip could effectively detect genetically modified rice with high specificity and the sensitivity limit was 0.1％.This chip can be used by the entryexit inspection and quarantine authorities and be applied for food safety testing.

关 键 词：酶连接探针杂交芯片 转基因水稻 品系检测 

分 类 号：Q783[生物学—分子生物学]