氢气饱和生理盐水对内毒素诱导的小鼠急性肺损伤的早期保护作用  

Early protective effect of hydrogen-saturated saline on lipopolysaccharide induced acute lung injury in mice

作　　者：毛蕾蕾[1] 罗燮 罗自强[3] 

机构地区：[1]中南大学湘雅二医院儿科,长沙市410000 [2]中南大学湘雅二医院内分泌科,长沙市410000 [3]中南大学基础医学院生理教研室,长沙市410003

出　　处：《实用医学杂志》2015年第1期14-16,共3页The Journal of Practical Medicine

基　　金：国家大学生创新创业训练计划项目(编号:AY12043)

摘　　要：目的:探讨氢气饱和生理盐水对内毒素(LPS)诱导的小鼠急性肺损伤(ALI)的早期保护作用。方法:将小鼠随机分为正常对照组、氢气饱和生理盐水对照组(H2组)、内毒素损伤组(LPS组)、氢气饱和生理盐水治疗组(LPS+H2组),1或2 h后检测肺湿干重比、肿瘤坏死因子α(TNF-α)和白细胞介素-10(IL-10)m RNA表达量。结果 :与正常对照组相比,LPS组的湿干重比显著增加(P<0.01),TNF-αm RNA表达量持续增加(P<0.01),IL-10 m RNA表达量在2 h时才增加(P<0.05)。与LPS组同一时间相比,LPS+H2组湿干重比降低(P<0.05),TNF-αm RNA表达量先降低(P<0.01),后差异无显著性(P>0.05),IL-10 m RNA表达量差异均无显著性(P>0.05)。结论:LPS诱导的ALI早期,腹腔注射氢气饱和生理盐水能够抑制促炎因子的早期表达,从而减轻内毒素诱导的小鼠急性肺损伤。Objective To evaluate the early protective effect of hydrogen-saturated saline on lipopolysaccharide （LPS） induced acute lung injury （ALI） in mice. Methods Mouse were randomly divided into sham group, the hydrogen-saturated saline control group （H2 group）, lipopolysaccharide injury group （LPS group） and hydrogen-saturated saline therapy group （LPS＋H2 group）. The lung wet/dry weight ratio, tumor necrosis factor alpha （TNF-α） and interleukin-10（IL-10） mRNA expression were measured after 1 hour or 2 hours. Results Compared with sham group, the wet/dry weigh ratio of LPS group significant increased （P 〈 0.01）, TNF-α mRNA expression increased （P 〈 0.05）, and IL-10 mRNA expression increased（P 〈 0.05） after 2 hours. Compared with LPS group at the same hour, the wet/dry weigh ratio of LPS＋H2 group decreased （P 〈 0.05）, TNF-α mRNA expression decreased （P 〈 0.01） after 1 hour. But the difierence was not significant alter 2 hours （P 〉 0.05）. The difference in IL-10 mRNA expression was not significant （P 〉 0.05）. Conclusion In the early stage of ALl induced by LPS, hydrogen-saturated saline could reduce the LPS-induced acute lung injury by inhibiting the early expression of inflammatory cytokines.

关 键 词：急性肺损伤 氢气水 内毒素 

分 类 号：R563.8[医药卫生—呼吸系统]