霍山石斛多糖的肠黏膜免疫调节活性及在小肠中的吸收分布  

Intestinal Mucosal Immunomodulating Activity of Polysaccharide from Dendrobium huoshanense and Its Absorption and Distribution in Small Intestine

作　　者：郝冉[1] 王正明[1] 查学强[1] 潘利华[1] 罗建平[1] 

机构地区：[1]合肥工业大学生物与食品工程学院、中草药与功能食品研究所,安徽合肥230009

出　　处：《食品科学》2014年第9期256-259,共4页Food Science

基　　金：安徽省科技攻关计划项目(12010402088);国家自然科学基金青年科学基金项目(21006019);国家自然科学基金面上项目(31271814)

摘　　要：目的 :研究霍山石斛多糖(Dendrobium huoshanense polysaccharide,DHP)的小鼠肠黏膜免疫调节活性以及在小肠内的吸收分布。方法:通过溴化氰活化法对DHP进行荧光标记,检测荧光标记前后的肠黏膜免疫调节活性;通过口服和离体小肠培养以及Peyer’s结细胞培养,用激光共聚焦显微镜检测DHP在小肠内的吸收分布以及与Peyer’s结细胞的结合。结果:DHP在质量浓度为25、50、100μg/mL和200μg/mL时,其肠黏膜免疫调节活性分别提高到对照组的112.8%、131.1%、137.6%和160.5%,荧光标记不改变DHP肠黏膜免疫活性;DHP在体内和体外均可被小肠吸收,分布于肠黏膜固有层内,并可与Peyer’s结内细胞结合。结论:DHP具有肠黏膜免疫调节活性,可以通过Peyer’s结细胞被小肠吸收,并分布于固有层内。Objective： To investigate the intestinal mucosal immunomodulating activity of a homogeneous Dendrobium huoshanense polysaccharide （DHP） and its absorption and distribution in the small intestine of mice. Methods： DHP was labeled with fuoresceinamine via cyanogen bromide activation, and the intestinal mucosal immunomodulating activity was detected. A confocal laser scanning microscope was used to detect the absorption of DHP and binding to Peyer＇s patch cells via oral administration, in vitro small intestine co-culture and co-culture with Peyer＇s patch cells. Results： The intestinal mucosal immunomodulating activity was increased by 12.8%, 31.1%, 37.6% and 60.5% when compared with the control at DHP concentrations of 25, 50, 100 and 200 ~g/mL, respectively. Fluorescent labeling had no effect on the intestinal mucosal immunomodulating activity of DHP. It could not only be absorbed through the small intestine in vivo and in vitro, but also could distribute in intestinal lamina propria and bind to certain cells in Peyer＇s patches. Conclusion： DHP possesses intestinal mucosal immunomodulating activity and can be absorbed through Peyer＇s patch cells of the small intestine.

关 键 词：霍山石斛 多糖 荧光标记 肠黏膜 分布 

分 类 号：Q94[生物学—植物学]