浅谈激光共聚焦显微镜使用技巧  

Discussion on the Using the Technique of Laser Confocal Microscope

作　　者：李叶[1,2] 黄华平[1,2] 林培群[1] 李勤奋[1,2] 杨腊英[1,2] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737

出　　处：《现代科学仪器》2014年第5期156-160,共5页Modern Scientific Instruments

基　　金：国家自然科学基金（项目编号：31301629）;中央级公益性科研院所基本科研业务费专项（项目编号：2012hzs1J003）;海南省重大专项（项目编号：ZDZX2013023-1-25）

摘　　要：本文简要介绍了激光扫描共聚焦（LSCM）成像原理,并以花粉为例,详细介绍了共聚焦针孔直径、光电倍增管检测器增益、激光强度、扫描速度、扫描方式、Z轴步距等重要参数设置对共聚焦成像的不同影响。探讨了正确使用LSCM的方法与技巧,如获取高质量的图像、图像保存及图像处理,以便为科技人员利用LSCM开展更多植物学与环境科学相关的研究提供参考。The imaging principle of Laser Scanning Confocal Microscopy （LSCM） was introduced in this paper, and the effect of some important parameters on confocal imaging were introduced by taking the pollen as an example, including confocal pinhole diameter, the gain of the photomultiplier tube detector, the intensity of the laser, scanning speed, scanning mode, Z-axis step. And also the methods and techniques for rightly using LSCM were introduced, such as how to get high-quality images, images saving and image processing.These results will provide references for the researchers to carry out more botany and environmental science-related research by using LSCM.

关 键 词：激光共聚焦扫描显微镜 花粉 针孔直径 激光强度 图像 

分 类 号：TH742.64[机械工程—光学工程]