温度对赤潮异弯藻生长速率及细胞体积和生化组成影响的研究  

Effect of Temperature on the Growth Rate,Cell Volume and Biochemical Composition of Heterosigma akashiwo Hada:An Experimental Study

作　　者：王燕[1,2] 宋洪军[1,3] 李艳[1,3] 李瑞香[1,3] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]海洋信息与计算中心,山东青岛266061 [3]海洋生态环境与工程国家海洋局重点实验室,山东青岛266061

出　　处：《中国海洋大学学报（自然科学版）》2015年第1期41-46,共6页Periodical of Ocean University of China

基　　金：国家重点基础研究发展计划项目(2010CB428701);海洋公益性行业科研专项(201005015);科技基础性工作专项(2008FY230600)资助

摘　　要：在实验室条件下,研究了温度对赤潮异弯藻生长速率、细胞体积和生化组成的影响.结果表明,赤潮异弯藻在10～30℃之间均能正常生长,25℃时生长速率最高,25℃为其最适生长温度;赤潮异弯藻的细胞体积随着温度的升高大致呈逐渐减小的趋势,10℃时细胞体积最大（823.89μm^3）,25℃时细胞体积最小（387.98 μm^3）.进一步分析表明,赤潮异弯藻生长速率与细胞体积（对数值）呈显著的负相关关系（P＜0.05）.在10～25℃之间,赤潮异弯藻单个细胞的碳、氮含量随着温度的升高呈逐渐减少的趋势,其单位体积细胞的碳、氮含量却呈单峰变化（15℃时结果为峰值）现象;叶绿素α含量在单个细胞和单位体积细胞两个层次上随温度的变化趋势一致,即其在整个温度区间内随着温度的升高均呈逐渐增加的趋势.赤潮异弯藻细胞C∶Chlα和N∶Chla随着温度的升高呈先减小后增加的趋势,均在10℃时最大,在25℃时最小.The effect of temperature on the growth rate,cell volume and cell biochemical composition of Heterosigma akashiwo was studied experimentally.The results showed that temperature ranging from 10 ℃ to 30 ℃ was suitable for the normal growth of H.akashiwo.The highest growth rate was observed at 25 ℃,which was considered as the optimum for the growth of H.akashiwo.Cell volume of H.akashiwo tended to decrease when temperature increased,reaching the maximum （823.89 μm^3） at 10 ℃ and touched the minimum （387.98 μm^3） at 25 ℃.Further analysis indicated that there was a negative correlation between lg（cell volume） and lg（growth rate） of H.akashiwo （P＜0.05）.When temperature increased from 10 ℃ to 25 ℃,it was found that cell carbon and nitrogen contents of H.akashiwo decreased significantly while cell carbon and nitrogen contents per unit volume of H.akashiwo changed in a singlepeak type and the peak was found at 15 ℃.However,cell chlorophyll a content and cell chlorophyll a content per unit volume both tended to increase with temperature.Results indicated that cell C ∶ Chl a and N ∶ Chl a of H.akashiwo decreased first and then increased,reaching the greatest at 10 ℃ and touched the least at 25 ℃.

关 键 词：赤潮异弯藻 温度 生长速率 细胞体积 生化组成 

分 类 号：Q945[生物学—植物学]