钼杂多酸光度法测定蔬菜中的锗  

Spectrophotometric Determination of Germanium in Vegetable with Crystal VioletHeteropoly Molybdogermaric Acid System in Nonionic Microemulsion Medium

作　　者：张莹琪[1] 刘铭[1] 马赫[1] 

机构地区：[1]太原工业学院,太原030008

出　　处：《食品工业》2015年第1期275-277,共3页The Food Industry

基　　金：山西省高校科技研发项目(No.20111028)

摘　　要：锗有一定的医疗保健功能,为了简便准确的测定锗含量,建立了在OP-10/正丁醇/正庚烷/水非离子型微乳液中,用结晶紫-锗钼杂多酸分光光度法测定样品中锗的新方法。优化的试验条件为4.8 mL 1.0 mol/L硫酸,2.0 m L2.5×10^-2 mol/L钼酸铵,1.1 m L微乳液,4.0 m L 2×10^-4 mol/L结晶紫。结晶紫-锗钼杂多酸形成的缔合物的最大吸收波长为650 nm,线性回归方程为y=0.143 2x＋0.022 2,锗含量在0.5~8μg/25 m L范围内符合比尔定律。方法的最低检出限为1.34×10^-4μg/m L,用于多种蔬菜中锗的测定,相对标准偏差为1.9%~2.4%,回收率97.1%~101.7%。Germanium has certain function of health care, in order to get simple and accurate determination of germanium, a novel spectrophotometric method of determination of germanium with crystal violet-heteropoly molybdogermaric acid system in nonionic microemulsion of OP-10/n-C4H9OH/n-C7H16/H2O is established. The optimal experimental conditions are 4.8 mL 1.0 mol/L H2SO4, 2.0 mL 2.5×10^-2 mol/L ammonium molybdate, 1.1 mL microemulsion, 4.0 mL 2×10^-4 crystal violet. The maximum absorption wavelength of the crystal violet with germanium-molybdenum heteropoly acid association complex is 650 nm. Linear regression equation is y=0.143 2x＋0.022 2 and Beer＇s law is obeyed in the range of 0.5-8.0 μg/25 mL. The detection limit is 1.34×10^-4 μg/mL. The method has been applied to the determination of germanium in vegetables. The RSD is 1.9%~2.4% and the recovery rate is 97.1%-101.7%.

关 键 词：微乳液 锗 分光光度法 测定 

分 类 号：O657.3[理学—分析化学] TS255.7[理学—化学]