某院宫颈疾病患者人乳头瘤病毒感染及分型情况  

Human papillomavirus infection and typing situation in cervical disease patients in a hospital

作　　者：朱振坤 徐亚丽 薛静俊 胡荣盛 

机构地区：[1]浙江省宁海县第一医院,浙江宁海315600

出　　处：《上海预防医学》2015年第1期16-19,共4页Shanghai Journal of Preventive Medicine

摘　　要：[目的]应用流式荧光杂交法检测人乳头瘤病毒（HPV）,分析宫颈疾病患者HPV感染及分型情况。[方法]以2013年1月至2013年12月浙江省宁海县第一医院4 041例宫颈疾病患者作为研究对象,采集宫颈脱落细胞,用流式荧光杂交技术检测HPV基因型,并对HPV感染率、基因亚型、感染类型和年龄分布等进行分析。[结果]检出HPV感染744例（18.41%）,其中单一亚型感染603例（14.92%）,多重亚型感染141例（3.49%）。可供检测的26种HPV基因亚型均有检出,最常见的亚型为HPV-16、52、58、39、18、61、59、6等,其中低危型以HPV-61、6、11亚型感染为主。高危型以HPV-16、52、58亚型感染为主。HPV感染亚型以高危亚型为主,单一高危亚型感染阳性率13.07%（528/4 041）,多重高危亚型感染阳性率2.15%（87/4 041）。31~40岁妇女HPV感染阳性率最高,阳性率22.22%,50岁以上者HPV感染阳性率最低,阳性率13.99%,且各年龄组中均以高危亚型感染检出率最高。[结论]宫颈疾病患者中HPV感染率较高,基因型分布较广,流式荧光杂交法是检测HPV感染的快速有效方法,HPV分型检测对宫颈疾病的防治意义重大。Objective To investigate HPV infection and its typing situation in cervical disease patients in a hospital by flow fluorescence hybridization for detection of human papillomavirus （HPV）. [Methods] From January to December 2013, a total of 4 041 cervical disease patients were as research objects.The cervical exfoliated cell specimens were collected and HPV genotypes were detected by flow fluorescence hybridization , while HPV infection rate , genetic subtypes , infection type and age distribution were analyzed . [ Results ] HPV infection was found in 744 cases （ 18 .41%） , in which 603 cases （14.92%） were single subtype and 141 cases（3.49%） multiple subtypes of infection.All 26 kinds of HPV subtypes available to detection were detected .The common subtypes were HPV-16, 52,58,39,18, 61,59,6 etc., in which HPV-61,6,11 subtypes were with low-risk and HPV-16,52,58 subtypes with high-risk.More common type of infection was high-risk subtype , in which single high-risk subtype infection was 13.07%（528/4 041） and multiple high-risk subtypes infection 2.15%（87/4 041）.The highest rate of HPV infection（22.22%） was found in the women 31 to 40 years of age, and the lowest（13.99%） in those above 50 years.In all age groups , the high-risk subtype infection was found with the highest detection rate . [ Conclusion] Patients with cervical disease are found to have higher HPV infection rate with wider distribution genotype .Flow fluorescence hybridization proves a fast and effective method to detect HPV infection, and HPV genotyping has significance for prevention and treatment of cervical disease .

关 键 词：人乳头瘤病毒 基因型 感染率 流式荧光杂交法 宫颈脱落细胞 

分 类 号：R737.33[医药卫生—肿瘤]