建立膀胱癌细胞系BIU-87原位移植瘤模型的实验研究  

Experimental study for establishment of orthotopic transplantation tumor model of human bladder cancer cell line BIU-87

作　　者：闫三华 杨晓峰[2] 刘杰昊 张自强[1] 张帆[3] 罗俊茜 庞建智 王玮[1] 

机构地区：[1]山西医科大学,山西太原030001 [2]山西医科大学第一医院泌尿外科,山西太原030001 [3]山西医科大学免疫教研室,山西太原030001

出　　处：《国际泌尿系统杂志》2015年第1期1-3,共3页International Journal of Urology and Nephrology

基　　金：国家自然科学基金项目资助（编号：81172444）

摘　　要：目的 探讨建立人体膀胱癌细胞系BIU-87膀胱原位移植瘤模型的有效方法,为膀胱癌实验动物的相关研究提供理论基础.方法 BALB-C系裸鼠20只,分成A、B两组（各10只）,A组采用手术方法于膀胱壁内注射人体膀胱癌细胞系BIU-87,B组经膀胱灌注人体膀胱癌细胞系BIU-87,分别观察不同阶段两组裸鼠膀胱肿瘤的发生发展情况.实验终点（4周）处死裸鼠,进行瘤体大小及组织学检测.结果 A组裸鼠中,一只于术后第2d死亡,其余存活9只中,8只成瘤,成瘤率为89％;B组10只均未成瘤.结论 经膀胱灌注不能建立理想的膀胱原位移植瘤模型,手术方法建立膀胱原位移植瘤模型的效果确切,能较好地模拟人膀胱肿瘤的疾病发展过程,对国人膀胱肿瘤的研究具有一定应用价值.Objectives To investigate the effective method of establishing bladder Orthotopic transplantation tumor model with human bladder cancer cell line BIU-87 to provide theoretical basis for the study of experimental animals of bladder cancer.Methods 20 BALB-C nude mice were divided into group A and B （10 PCs）,group A were injected with human bladder cancer cell line BIU-87 into the bladder wall through surgical method,B group were instilled with human bladder cancer cell line BIU-87 through bladder,progression of bladder tumor of the two groups was respectively observed in different stage.All mice were executed at experimental endpoint（week 4）,tumor size and histological detection were evalued.Results A mouse of group A died on the first day after operation,tumor occured in 8 of the rest surviving mice,tumor formation rate was 89% ; no tumor occured in B group.Conclusions Ideal orthotopic transplantation tumor model cannot established through bladder Instillation,surgery method to establish Orthotopic transplantation tumor model can definitely simulate development process of human bladder tumor disease better,which enjoy applicational value on the study of Chinese bladder tumors.

关 键 词：膀胱肿瘤 细胞系 肿瘤 疾病模型 动物 

分 类 号：R737.14[医药卫生—肿瘤]