萃取结晶法富集大豆油脚中的辅酶Q_(10)  

Enrich Coenzyme Q_(10) from Soybean Oil Stocks by Extractive Crystallization Method

作　　者：李朝艳[1] 

机构地区：[1]南阳理工学院生物与化学工程学院,河南南阳473004

出　　处：《大豆科学》2014年第6期920-923,共4页Soybean Science

摘　　要：利用萃取结晶法从大豆油脚中富集辅酶Q10,通过高效液相色谱法对分离产物进行了检测,考察了萃取过程中正己烷、丙酮、乙醇、水用量以及结晶温度和时间对辅酶Q10回收率和纯度的影响。结果表明:最佳的工艺条件为100 g的大豆油脚,加300 m L正己烷分2次进行萃取,过滤。滤液加0.5 g Na Cl盐析,分层,取油相旋转蒸发浓缩,回收正己烷,浓缩液中加100 m L丙酮分2次萃取,过滤,滤饼回收卵磷脂,滤液旋转蒸发,回收丙酮,油相加入100 m L95%乙醇溶解,加入20 m L H2O,0℃结晶6 h。在该工艺条件下,辅酶Q10的回收率达到93.30%,纯度达到73.31%。In this study,coenzyme Q10 were enriched from soybean oil stocks by extractive crystallization method and the production were tested by HPLC. The suitable condition was： use 300 mL n-hexane dissolve 100 g soybean bottom oil twice,filtrate it and add 0. 5 gNa Cl,separate organic phase,rotary evaporation to remove n-hexane,use 100 m L acetone extraction concentrate twice,filtrate,recycling lecithin from filter cake,rotary evaporation to remove acetone from liquid,mix 100 mL 95% ethanol to liquid,add 20 mLH2 O,put mix liquid at 0℃,collect crystallization after 6 h. The results showed that the coenzyme Q10＇s purity was 73. 31%,yield was 93. 30% under the optimal condition.

关 键 词：大豆油脚 辅酶Q10 萃取 结晶 

分 类 号：TQ464.8[化学工程—制药化工]