大叶蛇葡萄蛋白质组双向电泳分析  

Proteome Analysis of Ampelopsis Megalophylla by 2-DE

作　　者：黄静[1] 桂春[1] 答国政 夏叶[1] 程佩佩[1] 张秀桥[1] 

机构地区：[1]湖北中医药大学药学院,武汉430065

出　　处：《世界科学技术-中医药现代化》2014年第11期2380-2384,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上项目(31170335):大叶蛇葡萄降压药效作用的物质机制及蛋白表达研究;负责人:张秀桥;湖北省自然科学基金委重点项目(2014CFA083):基于转录组学的大叶蛇葡萄活性成分生物合成分子机制研究;负责人:张秀桥

摘　　要：目的:利用双向电泳法研究大叶蛇葡萄活性成分蛋白表达。方法:采用Tris饱和酚提取和醋酸铵甲醇沉淀大叶蛇葡萄嫩叶中的蛋白质;运用双向电泳法分离蛋白质,并对扫描后的二维凝胶电泳图谱进行分析,得到蛋白质表征图谱。结果:根据各个样品间蛋白点灰度值的比值差异,选择了差异较大的7个蛋白点进行质谱鉴定,鉴定了其中两种蛋白,分别为Hypothetical Protein和Unnamed Protein Product。结论:双向电泳技术的应用为从分子水平研究大叶蛇葡萄活性成分蛋白表达奠定了良好的基础。This study was aimed to investigate active ingredients from A mp e lop s is me galop hylla of protein expression by using two-dimensional gel electrophoresis （2-DE）. Methods： isn this study, proteinsfrom the tender leaves of A mpelopsis megalophylla were precipitated by Tris-phenol extraction and ammonium acetate methanol precipitation. Then using 2-DE technology to isolate protein.Finally, 2-DE was analyzed by the scanner, and got protein characteristic maps. Result：According to the differences in ratio of gray value, the seven differential protein points were chosen to identify the mass spectrum. At present, two proteins were identified, namely the hypothetical protein and the unnamed protein product. Conclusion： the application of 2-DE technology for A mpelopsis megalophyllathe active ingredient of protein expression at the level of molecular research laid a good foundation.

关 键 词：大叶蛇葡萄 蛋白质组 蛋白质提取 双向电泳 

分 类 号：R284.1[医药卫生—中药学]