MTHFR基因多态性与中国北方人群脑膜瘤发病的相关性分析  

Correlative Analysis of MTHFR Gene Polymorphism with the Incidence of Meningioma in Population of North China

作　　者：陈明辉[1] 李瑞岩[1] 冯岩[1] 蒿忠飞 赵哲峰[1] 孙旭[1] 李永利[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经外科,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2014年第35期6827-6829,共3页Progress in Modern Biomedicine

基　　金：黑龙江省青年科学基金项目(QC2014C108)

摘　　要：目的:探讨亚甲基四氢叶酸还原酶(MTHFR)基因多态性与中国北方人群脑膜瘤发病的相关性。方法:选择2012年1月-2013年12月在黑龙江省哈尔滨医科大学附属第二医院的第一、三病房接受手术治疗的脑膜瘤患者317例(实验组)及320例非脑膜瘤患者(对照组)为研究对象,利用聚合酶链反应限制性多态性片段长度(PCR-RFLP)检测和比较两组MTHFR两个单核苷酸多态性位点(C677T、A1298C)各种基因型(CC、CT、TT)的分布情况及等位基因的频率。结果:两组MTHFR的C677T中CC基因型的频率和TT基因型的频率比较有显著性差异(CC:OR=2.012,95%CI=1.460-2.772;TT:OR=0.399,95%CI=0.254-0.628,P<0.05),实验组MTHFR的(0.450)C677T中的T等位基因频率明显高于对照组(0.320)(OR=0.529,95%CI=0.420-0.666,P<0.05)。两组A1298C的等位基因分布比较没有统计学差别(P>0.05)。结论:MTHFR基因的C677T中TT等位基因提示潜在的易患脑膜瘤的风险,而CC等位基因会降低中国北方人群患脑膜瘤的风险。Objective： To explore the correlation of gene polymorphism of msethylene tetrahydrofolate reductase （MTHFR） with the incidence of meningiomain in a Han population of north China. Methods： A total of 317 cases of meningioma patients （experimental group） and 320 cases of non-meningioma patients （control group） were enrolled in this case-control study. The patients who underwent surgery were recruited from the First and Third Ward of Neurosurgery of the Second Affiliated Hospital of Harbin Medical University, Heilongjiang Province, North of China from January 2012 to December 2013. The distribution of different genetypes （CC, CT, TT） and allele frequencies of two SNPs （C677T and A1298C） of MTHFR were detected and compared between two groups using polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP）. Results： There were statistically significant differences in the CC and TT （CC： OR=2.012, 95% CI=1.460-2.772; TT： OR=0.399, 95% CI=0.254-0.628, P〈0.05） frequencies of MTHFR C677T between two groups （P〈0.05）. The T allele fi＇equency of MTHFR C677T of experimental group （0.450） was significantly higher than that of the control group （0.320） （OR=0.529, 95% CI=0.420-0.666, P〈0.05）. No statistically significant difference was observed in the allele distribution of A1298C between two groups （P〉0.05）. Conclusion： TT genotype of MTHFR C677T indicated a potential risk of meningioma, while the decrease of CC alleles of C677T reduced the risk ofmeningioma in a Han population in northern China.

关 键 词：亚甲基四氢叶酸还原酶 脑膜瘤 多态性 关联性 

分 类 号：R739.4[医药卫生—肿瘤]