ABCG2与胶质瘤血管形成及预后的相关研究  

Correlative Research of ABCG2 with Angiogenesis and Prognosis in Patients of Glioma

作　　者：肖宏[1] 胡炜[1] 张涵[1] 陈赤丹[2] 王来藏[1] 任付宾[1] 王宏伟[1] 

机构地区：[1]哈尔滨医科大学第四临床医学院微创神经外科,黑龙江哈尔滨150001 [2]哈尔滨医科大学第四临床医学院介入科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2014年第35期6916-6922,共7页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12511257)

摘　　要：目的:研究ABCG2在胶质瘤血管形成过程中与VEGF、VEGFR和CD34的关系,探讨其在血管形成中的作用及对胶质瘤患者生存预后的影响。方法:采用脑胶质瘤的组织芯片技术,分析ABCG2、VEGF和VEGFR(flt-1)在胶质瘤中的表达率,根据CD34阳性的血管计数判定微血管密度(MVD);另用免疫荧光共聚焦检测ABCG2与CD34、VEGF的共表达;用COX回归模型分析ABCG2对胶质瘤患者预后影响。结果:ABCG2、VEGF、VEGFR(flt-1)和CD34阳性表达率随着胶质瘤恶性程度的增加而增高。ABCG2Ⅰ、Ⅱ级之间无统计学差异,其余各级别之间存在统计学差异(P<0.05);ABCG2与病理级别呈正相关;ABCG2表达水平与MVD显著相关,γ=0.540,P<0.001。ABCG2、VEGF和VEGFR(flt-1)均为阳性表达的肿瘤标本MVD平均值显著高于ABCG2阴性表达者,P<0.001。ABCG2与CD34、VEGF共表达于血管壁。COX回归模型证明ABCG2是胶质瘤患者预后的危险因素。结论:ABCG2阳性表达细胞具有向肿瘤血管细胞分化的潜能,对肿瘤血管研究重要意义,且ABCG2表达可作为考察胶质瘤患者预后的重要指标。Objective： To study the relation of ABCG2 in glioma angiogenesis with VEGF, VEGFR and CD34, and investigate its role in angiogenesis, and to analyze its effect on prognosis of glioma patients. Methods： A tissue microarray technology of glioma was used to analyze the rate of ABCG2, VEGF and VEGFR（flt-1） expression in glioma. According to the numbers of CD34 positive labeled microvasculars, tumor microvasculars density（MVD） was calculated. The immunofluorescence confocal was used to detect the coexpression of ABCG2 and CD34, VEGF in the vessel wall. COX regression model was used to analyze the relation between ABCG2 and prognosis of patients with glioma. Results： The positive expression rate of ABCG2, VEGF, VEGFR and CD34 increased with the pathological grades. The expression of ABCG2 was statistically different（P〈0.05） among each pathological grades except the grade Ⅰ-Ⅱ. The expression of ABCG2 and pathological grades was positively correlated; moreover, the expression level of ABCG2 was intimately related with MVD in tumors, γ=0.540, P〈0.001. The MVD in gliomas of grade Ⅲ-Ⅳ was higher than that in grade Ⅰ-Ⅱ.The genes of VEGF, VEGFR（flt-1） and CD34 were also analyzed, showing the average value of MVD in tumors expressing VEGF＋,VEGFR＋（flt-1） and ABCG2＋was significantly higher than that in the ABCG2-tumors（P〈0.001）. ABCG2 was co-expressed with CD34 and VEGF in the vessel wall. COX regression model showed that ABCG2 could be as a prognostic factor for the patients with glioma.Conclusions： ABCG2 positive cells have potential to differentiate into the tumor vascular cells, which would be significant for the study of tumor blood vessels. Furthermore, ABCG2 expression may be an important prognostic indicator in patients with glioma.

关 键 词：脑胶质瘤 组织芯片 免疫组化 预后 

分 类 号：R739.4[医药卫生—肿瘤]