新疆乳腺癌患者外周血中p16基因甲基化的检测意义  

The significance of the detection of p16 gene methylation in peripheral blood in Xinjiang breast cancer patients

作　　者：李莉[1] 鲁英[1] 杨晓燕[1] 欧江华[2] 齐新[2] 

机构地区：[1]新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室,新疆乌鲁木齐830011 [2]新疆医科大学附属肿瘤医院乳腺外科,新疆乌鲁木齐830011

出　　处：《东南大学学报（医学版）》2014年第6期691-696,共6页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81160137);新疆维吾尔自治区教育厅青年教师科研培育基金资助项目(XJEDU2011S23)

摘　　要：目的:探讨新疆乳腺癌患者p16基因甲基化的情况。方法:按照1∶1配比的病例对照研究,采用聚合酶链反应(PCR)对54对研究对象外周血中p16基因进行扩增后焦磷酸测序,检测该基因的甲基化情况。结果:经单因素分析,p16基因各基因位点甲基化程度差异没有统计学意义(P>0.05)。根据ER阴性阳性分组,PM00139972Cp G-2的甲基化程度差异有统计学意义(P<0.05);根据HER-2阴性阳性分组,PM00139972Cp G-4的甲基化程度差异有统计学意义(P<0.05)。结论:对新疆乳腺癌患者p16基因各位点的检测结果表明,其异常甲基化对乳腺癌的预后可能有意义。Objective：To investigate the methylation of p16 gene in patients of breast cancer in Xinjiang.Methods：1∶1 case-control was adopted and the tumor suppressor gene p16 was amplified by polymerase chain reaction（PCR） in the peripheral blood of 54 pairs of subjects,and followed the pyrosequencing to detect the methylation of the gene.Results：Univariate analysis showed that the difference of methylation degree of each p16 gene was not statistically significant.The degree of methylation difference of PM00139972CpG-2 was statistically significant according to ER-negative-positive group; and the degree of methylation difference of PM00139972CpG-4 was statistically significant according to HER-2-negative-positive group.Conclusion：The abnomal mathylation of each p16 gene in Xinjiang breast cancer patients may be meaningful for the prognosis of breast cancer.

关 键 词：乳腺癌 P16基因 甲基化 焦磷酸测序 

分 类 号：R737.9[医药卫生—肿瘤]