曲酸对受辐射中国仓鼠卵巢细胞的保护作用  

Protective effect of kojic acid on Chinese hamster ovary cells from radiation damage

作　　者：李鹏飞[1,2] 王恺[2] 韩春光[2] 杜丽[2] 原美茹[2] 葛鹏新 尹力[2] 蔡文臣 刘超[2] 刘永学[2] 

机构地区：[1]安徽医科大学研究生学院,安徽合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《癌变．畸变．突变》2014年第6期438-440,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的：探讨曲酸对受辐射中国仓鼠卵巢细胞（CHO）的保护作用。方法：CHO细胞分别经不同强度（0-20 Gy ）^60Coγ射线照射，于照射后24、48和72 h采用MTT法检测细胞存活情况；细胞经不同浓度（分别为0、0.01、0.1、1、10、100μg/mL）曲酸作用1.5 h，采用12 Gyγ射线照射，于照射后24、48和72 h采用MTT法检测细胞存活情况；细胞经浓度为1μg/mL的曲酸作用1.5 h，分别经不同强度（6、12、16 Gy）γ射线照射，于照射后24 h采用MTT法检测细胞存活情况。结果：与对照组比较，γ射线照射导致CHO细胞的存活率下降（P均〈0.01），且随着照射强度的增加，该效应增强；曲酸在0.01-10μg/mL浓度范围内能明显提高12 Gy辐射条件下CHO细胞的存活率，其中浓度在1μg/mL时效应最明显；1μg/mL曲酸均能明显提高6、12、16 Gyγ射线照射下细胞的存活率。结论：曲酸明显提高受辐射CHO细胞的存活率，以保护细胞免受辐射损伤。OBJECTIVE： To explore the protection of kojic acid （KA） on CHO cells from radiation. METHODS：MTT assay was used to detect cell viability in the following conditions：CHO cells were exposed to different 60intensities （0-20 Gy） of ^60Coγray,and were cultured for 24,48 and 72 h. CHO cells were treated with KA （0、0.01、0.1、1、10、100μg/mL,respectively） for 1.5 h before exposure to 12 Gyγrays,and were cultured for 24,48 and 72 h,respectively;CHO cells were treated with KA at 1μg/mL for 1.5 h prior to exposed to 6,12 and 16 Gyγray,then were cultured for 24 h. RESULTS：CHO cell viability was decreased when they were exposed toγray in a dose-response manner. Cell viability was significantly increased when treated with 0.01-10 μg/mL KA before exposure to 12 Gy γray,with a peak effect at 1 μg/mL. Pretreatment of 1 μg/mL KA could significantly improve the viability of CHO cells exposed to 6,12,16 Gyγrays. CONCLUSION：Pretreatment with KA significantly improved the viability of irradiated CHO cells by its protective effect from radiation damage.

关 键 词：曲酸 CHO细胞 ^60Coγ射线 辐射损伤 保护作用 

分 类 号：Q345.7[生物学—遗传学]