应用显性致死试验检测西番莲果汁的遗传毒性  

Genotoxicity of passiflora fruit juice in mice using dominant lethal assay

作　　者：胡培丽[1] 林飞[1] 单纯[1] 赵强[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《癌变．畸变．突变》2014年第6期454-458,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的：应用小鼠显性致死试验评价西番莲果汁的遗传毒性。方法：西番莲果汁7.2-28.8 g/kg剂量对NIH种雄性小鼠连续灌胃染毒5 d，再与雌性小鼠连续同笼交配7周，每批雌性小鼠在妊娠第13-14天处死，观察胚胎着床数、活胎数和死胎数。同时设置对照组。对所有计数、计量资料进行统计学处理。结果：环磷酰胺（CP）和丝裂霉素C（MMC）阳性对照小鼠除平均着床数正常外，其余各项指标在不同周次中均存在异常（P〈0.05或〈0.01）。阴性对照组和西番莲果汁各剂量组的小鼠交配率〉133%，妊娠率在60%-93%，窝平均着床数〉8.6只，平均死胎数〈0.8只，平均活胎数〉8.1只，均在正常数值范围内；各剂量西番莲果汁均未诱发有死胎孕鼠率、显性致死率和突变指数的增高及着床卵生存率的降低（P〉0.05）。结论：西番莲果汁在本试验剂量（7.2-28.8 g/kg）范围内，小鼠显性致死试验为阴性结果，提示其无遗传毒性。OBJECTIVE：In order to evaluate the genetic toxicity of passiflora fruit juice,the mice dominant lethal test was conducted. METHODS：Male NIH mice were gavaged for 5 consecutive days with 7.2-28.8 g/kg passiflora fruit juice,with positive and negative controls. Then individual males were mated sequentially at predetermined intervals th thwith two virgin females. Females were sacrificed on the 13 -14 day of pregnancy and the number of embryo implantated,live births and stillbirths were recorded. All data were evaluated by appropriate statistical methods. RESULTS：For cyclophosphamide and mitomycin C groups,all indicators in different weeks were abnormal in addition to the number of nidation（P〈0.05 or P〈0.01）. For negative and treated groups,mating rate （〉133%）,pregnancy rate （60%-93%）,number of nidation （〉8.6）,average number of stillbirths （〈0.8） and live births （〉8.1） were all in the normal ranges. There were no significant increase in stillbirth rate,dominant lethality and the mutation index nor reduction in fertilized survival of eggs （P〉0.05）. CONCLUSION：7.2-28.8 g/kg passiflora fruit juice did not show a positive response in mice dominant lethal test,indicating that the possibility of genetic toxicity was small.

关 键 词：显性致死试验 西番莲 小鼠 遗传毒性 

分 类 号：R394.6[医药卫生—医学遗传学]