EPC/CH-茶多酚柔性脂质体的制备研究  

Research on the Preparation of Tea Polyphenols-Loaded Flexible Liposomes

作　　者：禤智东[1] 黄岳山[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《中国医学物理学杂志》2014年第6期5333-5337,共5页Chinese Journal of Medical Physics

摘　　要：目的:应用响应面法优化茶多酚柔性脂质体的制备工艺参数,筛选出最佳处方。方法:以蛋黄卵磷脂(EPC)和胆固醇(CH)作为膜材,以胆酸钠作为柔软剂,采用逆向薄膜蒸发法制备茶多酚柔性脂质体。以脂质体的包封率为考察指标,采用三因素三水平Box-Behnken响应平面法考察脂质体膜材料中蛋黄卵磷脂(EPC)与胆固醇(CH)的质量比(A),蛋黄卵磷脂(EPC)与茶多酚(TP)的质量比(B),水油相的体积比(C)对影响茶多酚柔性脂质体包封率的影响,对结果进行二次多项式拟合,并根据模型优化处方,得到最优结果并进行验证。结果:确定最佳工艺条件为:卵磷脂与胆固醇比例为3.82:1,卵磷脂与茶多酚比例为2.68:1,油水相体积比为4.34:1,优化得到实际最优处方包封率为57.37%,实测值与理论值无显著性差异。结论:响应平面法用于茶多酚柔性脂质体处方的优化筛选是可行的,具有很好的预测性,此处方工艺稳定,重现性好,能制得较高包封率的茶多酚柔性脂质体。Objective： To optimize and prepare tea polyphenols-loaded flexible liposomes by Box-Behnken response surface methodology（RSM）. Methods： The liposomes using EPC and CH as membranous material, sodium cholate hydrate as softener ware prepared by reverse phase evaporation technique and optimized thought a three factor and three-level Box-Behnken, with the EPC： CH（A）, EPC： TP（B） and oil phase： water phase（C） as the independent variables, and the entrapment efficiency（Y） as the dependent variables.Second-order polynomial equations were fitted to the data, and the resulting equations were used to produce three dimensional response surface graphs, by which optimal experimental conditions were selected.Results： The optimal preparation conditions were determined as EPC/CH ratio 3.82：1, EPC/TP ratio 2.68： 1, oil phase/water phase ratio 4.34： 1. Under these conditions, the evaluated entrapment efficiencyof the liposomes was 57.37%. There was no significant difference between the measured value with the theoretical value.Conclusions： The response surface method is applicable for the formulation and preparation conditions of optimization ofteapolyphenols-loaded flexible liposomes, and the resulting optimal preparation technique is stable, feasible, and predictable.The liposomes with highly entrapment efficiencycan be prepared under optimal preparation conditions.

关 键 词：茶多酚 柔性脂质体 制备 性能 响应平面 

分 类 号：R318.08[医药卫生—生物医学工程]