大黄素对U937细胞凋亡及细胞周期相关基因的影响  

Effects of Emodin on Apoptosis and Cell Cycle related Genes in U937 Cells

作　　者：连晓岚[1] 胡建达[1] 郑志宏[1] 陈英玉[1] 

机构地区：[1]福建省血液病研究所,福建省血液病学重点实验室,福建医科大学附属协和医院,福建福州350001

出　　处：《中国实验血液学杂志》2014年第6期1535-1539,共5页Journal of Experimental Hematology

基　　金：福建省医学创新课题基金资助项目(2001CX02);国家及福建省临床重点学科建设项目;卫生行业科研专项项目合作经费(201202017);国家高技术研究发展计划(363计划)课题(2012AA02A505)

摘　　要：本研究旨在观察中药大黄素(Emodin)诱导人髓系白血病细胞株U937细胞的凋亡及凋亡时细胞周期相关基因的变化。采用逆转录聚合酶链反应(RT-PCR)检测60μmol/L大黄素作用前后U937细胞C-MYC、h-TERT、PIM-2、Survivin、野生型P53、P21、TG Fβ1、MCL-1等基因表达的变化。结果表明:随着大黄素作用时间的延长U937细胞C-MYC、h-TERT、PIM-2、Survivin基因表达逐渐减少,而野生型P53、P21、TGFβ1基因表达逐渐升高,MCL-1基因表达无明显改变。结论:大黄素诱导的U937细胞凋亡是多因素启动和网络调节的结果。This study was aimed to observe the effects of emodin on apoptosis and cell cycle related genes in human myeloid leukemia cell line U937 cells.U937 cells were exposed to 60 μ/L emodin for 24 h.The expressions of C-MYCh-TERTPIM-2Survivinwild type P53P21 TGF β1 and MCL-1 genes before and after treatment with emodin were determined and quantitated by using reverse transcriptase-polymerase chain reaction（ RTPCR）.The results showed that the expressions of C-MYCh-TERTPIM-2Survivin in treated U937 cells decreasedbut the expressions of WTp53P21 and TGFincreasedwhile the expression of MCL-1 gene had no obvious change.It is concluded that multiple pathways may be involved in the processes of emodin-induced U937 cell apoptosis.

关 键 词：U937细胞 大黄素 凋亡 RT-PCR 癌基因 抑癌基因 

分 类 号：R733.7[医药卫生—肿瘤]