雷帕霉素对顺铂作用下人肺腺癌A549及耐药A549/DDP细胞增殖、侵袭、黏附及自噬凋亡的影响  

Effect of rapamycin on proliferation,invasion,adhesion,apoptosis and autophagy of human lung adenocarcinoma A549 and resistant A549 / DDP cells treated with cis-diamminedichloroplatinum

作　　者：金柱[1] 高宝安[1] 

机构地区：[1]三峡大学第一临床医学院,宜昌市中心人民医院呼吸内科,湖北宜昌443003

出　　处：《中国病理生理杂志》2014年第12期2120-2127,共8页Chinese Journal of Pathophysiology

基　　金：湖北省自然科学基金资助项目(No.2011CDB178);湖北省教育厅中青年人才基金资助项目(No.Q20111202)

摘　　要：目的:研究雷帕霉素(Rap)对顺铂(DDP)作用下人肺腺癌A549及耐药A549/DDP细胞增殖、迁移、黏附及其自噬凋亡的影响。方法:培养人肺腺癌A549及耐药A549/DDP细胞株,利用MTT方法分别检测Rap和DDP单独与联合作用对A549及耐药A549/DDP细胞增殖抑制率的影响;Transwell方法检测Rap和DDP单独与联合作用对A549及耐药A549/DDP细胞体外侵袭能力的影响;黏附实验检测Rap和DDP单独与联合作用对A549及耐药A549/DDP细胞体外侵袭能力的影响;流式细胞术检测Rap和DDP单独与联合作用对A549及耐药A549/DDP细胞凋亡的影响;Western blotting检测Rap和DDP单独与联合作用对A549及耐药A549/DDP细胞自噬标志蛋白beclin-1和LC3表达的影响。结果:与Rap或DDP单独作用组相比,Rap和DDP联合作用能够同时显著抑制人肺腺癌A549及耐药A549/DDP细胞增殖、体外侵袭能力及细胞黏附能力,并能够促进细胞凋亡和自噬标志蛋白beclin-1和LC3的表达(均P<0.05)。结论:Rap能够通过促进细胞自噬而增强DDP的作用,进而抑制人肺腺癌A549及耐药A549/DDP细胞的增殖、侵袭、黏附并促进细胞凋亡作用,具有协同作用。AIM： To study the effect of rapamycin （Rap） on the proliferation, invasion, adhesion, apoptosis and autophagy of human adenocarcinoma A549 and resistant A549/DDP cells treated with cis-diamminedichloroplatinum （DDP）. METHODS： Human adenocarcinoma A549 and resistant A549/DDP cell lines were cultured. The inhibitory effects of Rap alone or combined with DDP on A549 and resistant A549/DDP cells were detected by MTY assay. The in vitro invasion abilities of the 2 cell lines treated with Rap alone or combined with DDP were detected by Transwell methods. The in vitro adhesion abilities of the 2 cell lines treated with Rap alone or combined with DDP were detected by adhesion experiments. The apoptosis of A549 and resistant A549/DDP cells induced by Rap alone or combined with DDP was ana- lyzed by flow cytometry. The cell autophagy marker proteins beclin-1 and LC3 in A549 and resistant A549/DDP cells trea- ted with Rap alone or combined with DDP were detected by Western blotting. RESULTS： Compared with Rap or DDP a- lone group, the combination of Rap and DDP significantly inhibited the proliferation, invasion and adhesion of A549 and resistant A549/DDP cells in vitro, and promoted the cell apoptosis and autophagy marker proteins beclin-I and LC3 expres- sion （ all P 〈 0. 05）. CONCLUSION ： Rap enhances the effect of DDP through promoting the cell autophagy, thereby in- hibiting the proliferation, invasion and adhesion of .4549 and resistant A549/DDP cells and inducing the cell apoptosis with a synergistic effect.

关 键 词：雷帕霉素 顺铂 人肺腺癌细胞 细胞凋亡 细胞侵袭 自噬 

分 类 号：R730.23[医药卫生—肿瘤]