人8-羟基鸟嘌呤糖苷酶1与年龄相关性白内障关系的临床研究  

Relationship between the altered expression of oxidative damage repair gene HOGG1 and age-related cataract

作　　者：李晨[1] 管怀进[2] 

机构地区：[1]苏州大学附属第一医院眼科,215000 [2]南通大学附属医院眼科,226001

出　　处：《临床眼科杂志》2014年第6期481-485,共5页Journal of Clinical Ophthalmology

基　　金：国家自然科学基金(No.81070718)

摘　　要：目的探讨与年龄相关性白内障(ARC)患者晶状体上皮细胞(LECs)中参与DNA损伤后碱基清除修复途径的氧化损伤修复基因—人8-羟基鸟嘌呤糖苷酶1(HOGG1)水平与ARC的关系。方法收集三种ARC(皮质性、核性、后囊下性)LECs样本,以透明晶状体LECs为对照组,用免疫组化、RT-PCR方法测定HOGG1在LECs的表达情况。结果对照组LECs中可见HOGG1的表达,三种ARC患者LECs中可见HOGG1表达较对照组增高(F=107.62,P<0.01),但三种ARC之间没有统计学差异。对照组与ARC组HOGG1均位于细胞质和细胞核。说明ARC LECs的细胞核和细胞质中HOGG1的表达量上调。结论 HOGG1表达上调参与ARC的发生发展。Objective To observe the altered expression of HOGG 1, a base excision repair enzyme for repairing oxidative DNA damage in anterior lens capsule epithelial cells （LECs） in age-related cataract （ARC） patients, and to ex-plore potential biological roles of HOGG 1 in the pathological mechanisms of ARC .Methods LECs samples of cortical cat-aract, nuclear cataract and posterior cataract were collected .Clear lens samples were collected as control .We conducted RT-PCR and immunochemistry to measure the expression of HOGG 1.Results We found that the expression of HOGG 1 was significantly higher in LECs of ARC patients than in control LECs （ F =107.62, P ＆lt;0.01）, but no differences be-tween cortical , nuclear or posterior subcapsular cataracts .Immunofluorescence showed that positive staining of HOGG 1 were seen in cell nucleus and cytoplasm in either group , but was stronger in cataractous LECs .Conclusion The mRNA and protein expression of HOGG 1 increased in ARC LECs , which may provide theoretical basis for early diagnosis and treatment of ARC.

关 键 词：年龄相关性白内障 8-羟基鸟嘌呤糖苷酶1 晶状体上皮细胞 

分 类 号：R776.1[医药卫生—眼科]