Gankyrin在甲状腺乳头状癌的表达及临床意义  

The Clinical Significance of Gankyrin Expression in Papillary Thyroid Carcinoma

作　　者：徐锦叶 沈沁彦 邢萍[1] 赖永锋[1] 金晓燕[2] 梁勇[2,3] 

机构地区：[1]温州医科大学第一临床学院,温州325000 [2]台州学院附属市立医院肿瘤外科,台州318000 [3]台州学院医学院,台州318000

出　　处：《中国细胞生物学学报》2014年第12期1661-1667,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金项目(批准号:81072209);2014年浙江省自然科学基金项目(批准号:Y14H160128);2012年卫生部医药卫生科技发展研究中心课题(批准号:2012FZ013);2012年浙江省医学会临床科研基金项目(批准号:2012ZYC-A100)资助的课题~~

摘　　要：采用荧光定量PCR、Western blot及免疫组化方法对比研究gankyrin m RNA及蛋白在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)、桥本氏甲状腺炎(Hashimoto’s thyroiditis,HT)及正常甲状腺组织中的表达情况,分析gankyrin蛋白表达与PTC临床病理特征的关系,探讨gankyrin基因对PTC发生发展的影响。结果显示,PTC及HT样本中gankyrin m RNA及蛋白水平的表达量均高于正常对照组,有统计学显著意义(P<0.01);PTC组及HT组间的表达无统计学差异(P>0.05);临床特征分析表明,gankyrin蛋白表达在PTC中的表达与患者的性别、年龄、TNM分期等无关,而与是否合并HT有关。Gankyrin基因在PTC及HT中的高表达说明其在这两种关系密切的疾病中扮演着重要的角色,提示其与PTC的分子机制有关。In this research, we evaluated the relationship between gankyrin expression and clinicopathologic parameters in papillary thyroid carcinoma （PTC）, exploring its effect on PTC using fluorescence quantitative PCR, Western blot and immunohistochemistry to investigate mRNA and protein levels of gankyrin in PTC, Hashimoto＇s thyroiditis （HT） and normal thyroid tissues. The results showed that both the mRNA and protein levels of gankyrin expression in PTC and HT were obviously higher than that in normal thyroid tissues （P〈0.01）, whereas the differ- ences of gankyrin expression were not significant between PTC and HT （P〉0.05）. In addition, gankyrin was over- expressed in PTC with coexisting of HT compared with PTC without HT. The high level ofganltyrin expression in both PTC and HT suggests that gankyrin plays an important role in the occurrence and development of these two closely related diseases and it is probably a molecular mechanism of PTC.

关 键 词：gankyrin 甲状腺乳头状癌 桥本氏甲状腺炎 

分 类 号：R736.1[医药卫生—肿瘤]