IL-8对膀胱癌T24细胞系干细胞特性维持调控的研究  

IL-8 can regulate the stemness in T24 bladder cancer cells

作　　者：朱凯[1] 崔迪[2] 韩邦旻[2] 

机构地区：[1]山东省苍山县人民医院泌尿外科,山东临沂277700 [2]上海交通大学附属第一人民医院泌尿外科,上海200080

出　　处：《中国现代医学杂志》2014年第36期14-18,共5页China Journal of Modern Medicine

摘　　要：目的从膀胱癌细胞系T24中分离CD44+的细胞,鉴定生物学特性后研究IL-8在膀胱癌CD44+干细胞中的作用价值。方法流式分选T24中CD44+细胞,稳定干扰CD44+T24细胞中IL-8,检测干细胞特征蛋白CD44、Oct4、Bmi1、Notch1、Sox2、Nanog、CK5和耐药基因ABCG2的表达;同时还检测癌细胞集落形成能力及其对阿霉素和长春新碱的耐药敏感性。结果干扰IL-8后,干细胞特征蛋白CD44、Oct4、Bmi1、Notch1、Sox2、Nanog、CK5和耐药基因ABCG2的表达显著降低,从而导致癌细胞成团生长能力明显下降,对化疗药物的敏感性增加。结论 IL-8对维持膀胱癌干细胞特性和抵抗化疗药物均密切相关,IL-8可能作为针对膀胱肿瘤干细胞的治疗靶点。[ Objective ] To separate CD44＋ stem cells from bladder cancer cell line T24 and identify the value of 1L-8 in maintenance its stem cell characteristics. [ Methods ] After sorting CD44~ and CD44- T24 cells, we detected the expression of Oct4, Bmil, Notehl, Sox2, Nanog, ABCG2, IL-8, CD44 and CK5 in IL-8 knocked down CD44＋ T24 cells as well as colony formation and drug resistance. [ Results ] After IL-8 knockdown, the expression of Oct4, Bmil, Notchl, Sox2, Nanog, ABCG2, IL-8, CD44, CK5 and colony formation, drug resistance were all decreased. [ Conclusions ] IL-8 can regulate the stemness in bladder cancer cells, which may be the target to bladder cancer stem cells.

关 键 词：肿瘤干细胞 膀胱癌 白细胞介素-8 

分 类 号：R737.14[医药卫生—肿瘤]