慢性氟中毒大鼠肝脏中P-糖蛋白和细胞核因子-κB的表达水平  

Expression Levels of P-glycoprotein and NF-κB in the Liver of Chronic Fluorosis Rats

作　　者：张凯琳[1] 吴昌学[2] 官志忠[1,2] 

机构地区：[1]贵阳医学院病理学教研室,贵州贵阳550004 [2]贵阳医学院分子生物学重点实验室,贵州贵阳550004

出　　处：《贵阳医学院学报》2014年第6期817-819,824,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金(81160335);科技部支撑计划课题(2013BAI05103);科技部国际合作项目(2010DFB30530);贵州省科学技术基金项目[黔科合J字(2012)2040号];贵州省科技厅项目[黔科合外Z字(2012)4010号];贵阳医学院院基金第020号

摘　　要：目的:研究慢性氟中毒大鼠肝脏中P-糖蛋白(P-glycoprotein,P-gp)和细胞核因子κB(Nuclear factorκB,NF-κB)的表达变化,探讨氟中毒肝脏损害的发病机理。方法:将60只SD大鼠随机分为3组,即对照组、小剂量染氟组和大剂量染氟组,分别给予自来水、加10 mg/L和50 mg/L氟(Na F)的的自来水喂养6个月观察3组大鼠氟斑牙程度及尿氟,同时取大鼠肝脏;采用蛋白印迹方法检测P-gp和NF-κB的蛋白表达水平,实时荧光定量PCR方法检测NF-κB mRNA表达水平。结果:6个月后,小剂量染氟组、大剂量染氟组大鼠氟斑牙程度及尿氟含量明显高于对照组,差异有统计学意义(P<0.05);小剂量染氟组、大剂量染氟组大鼠肝脏中P-gp和NF-κB蛋白和mRNA表达高于对照组,差异有统计学意义(P<0.05);小剂量染氟组、大剂量染氟组NF-κB mRNA表达高于对照组,差异有统计学意义(P<0.05)。结论:慢性氟中毒引起大鼠肝脏中P-gp和NF-κB表达明显升高,这可能与氟中毒性肝脏损伤的发生机制有关。Objective：To investigate the expressions of P-glycoprotein （G-pg） and nuclear factor κB （NF-κB） in the liver of chronic fluorosis rats,and to reveal the mechanisms of damaged liver resulted from the toxicity of the fluoride.Methods：The SD rats were randomly divided into three groups：control group,small dose fluoride exposure group and large dose fluoride exposure group.Three groups were fed with tap water,10 mg/L fluoride and 50 mg/L fluoride respectively for six months.Dental fluorosis degrees and urinary fluorine contents,and conditions of rats＇ livers were observed.The protein and mRNA expression levels of P-gp and NF-κB in livers were detected by Western blotting and quantitative real time PCR method.Results：Six months later,dental fluorosis degrees and urinary fluorine contents of small dose group and large dose group were remarkably higher than control group,differences were statistically significant （P ＜ 0.05） ; protein expression levels of P-gp and NF-κB in both fluoride exposure groups were significantly higher than those of control group,differences were statistically significant （P ＜ 0.05） ; NF-κB mRNA expression levels of both fluoride exposure groups were higher than those of control group,differences were statistically significant （P ＜ 0.05）.Conclusions：The increased expressions of P-gp and NF-κB in the liver of rat are induced by chronic fluorocsis,which is possible to correlate with the mechanism of liver damages triggered by fluoride poisoning.

关 键 词：氟中毒 大鼠 P-糖蛋白 细胞核因子ΚB 肝 

分 类 号：R361.2[医药卫生—病理学] R599.5[医药卫生—基础医学]