匹罗卡品癫痫模型中海马区TREK-2钾离子通道表达变化及意义  

Expression Changes and Meaning of TREK-2 Potassium Ion Channel in Hippocampus in Pilocarpin-induced Epilepsy Model

作　　者：高琴琴[1] 王晖[1] 刘明富[1] 江涛[1] 刘昕乐 肖昭扬[3] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710032 [2]第四军医大学西京医院超声科,陕西西安710032 [3]大连医科大学第二附属医院急诊科,辽宁大连116000

出　　处：《现代生物医学进展》2015年第3期449-452,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81071052;81471373)

摘　　要：目的:研究匹罗卡品癫痫模型中海马区TREK-2双孔钾离子通道的表达变化,初步探讨TREK-2在癫痫发病过程中的机制及意义。方法:选用成年雄性SD大鼠腹腔注射氯化锂-匹罗卡品(lithium-pilocarpine)构建癫痫模型,分别在癫痫持续状态(status epilepticus,SE)后不同时间点(6 h、1 d、3 d、1 w、2 w、4 w、8 w)提取海马组织,利用western-blot检测海马区TREK-2随时间表达变化。并用TREK-2 si RNA下调海马区TREK-2表达,进一步观察对大鼠癫痫状态的影响。结果:与对照组相比,TREK-2在诱导癫痫持续状态发作后的3d开始降低(P<0.05),1 w,2 w,4 w明显降低(P<0.01),8 w时仍维持在很低水平(P<0.001)。在TREK-2表达下调后,大鼠癫痫潜伏时间(latent period)明显缩短,癫痫持续状态1 h 5级以上发作频率(seizure frequency)明显增加。结论:TREK-2在氯化锂-匹罗卡品致痫大鼠海马组织中表达的降低,且其下调加重癫痫状态的事实提示TREK-2参与了癫痫的发生发展过程。Objective： To investigate the expression change of K2P channel TREK-2 in hippocampus with pilocarpin-induced epilepsy, and to explore the mechanism and siginificance of epilepsy in which TREK-2 was involved. Methods： The epilepsy model was conducted by celiac injection of lithium-pilocarpine with adult male Sprague-Dawley rats, the hippocampus tissue was collected respectively at 6 h, 1 d, 3 d, 1 w, 2 w, 4 w and 8 w after the status epilepticus （SE）, and the expression changes of TREK-2 in hippocampus was detected at different time points with Western-Blot. The expression of TREK-2 was down-regulated in hippocampus with TREK-2 siRNA, and the effect on status epilepticus （SE） was further observed. Results： Compared to control group, the expression of TREK-2 began to decrease at 3 d （P 〈 0.05）, decreased significantly at 1 w, 2 w, 4 w, （P〈 0.01）, and remained a very low level at 8 w （P 〈 0.001）. After the expression of TREK-2 declined, the latent period of epilepsy decreased significantly, and the seizure frequency of SE significantly increased within 1 h above magnitude 5. Conclusion： The decreased expression of TREK-2 potassium ion channel in hippocampus in pilocarpin-induced epilepsy model and the down-regulated TREK-2 which aggravated the status epilepticus indicated that TREK-2 participated in the ocurrence and development of epilepsy.

关 键 词：TREK-2 氯化锂-匹罗卡品 癫痫 

分 类 号：Q-33[生物学] R742.1[医药卫生—神经病学与精神病学]