RNA干扰沉默MUC1-C对前列腺癌PC3细胞增殖的影响  

Effects of silencing MUC1-C with RNA interference on proliferation of human prostate cancer PC3 cells

作　　者：李静[1] 周建甫[2] 张秋红[1] 汪茜[1] 韩守威 陈志强[2] 向松涛[2] 

机构地区：[1]广州中医药大学第二临床医学院,510006 [2]广东省中医院泌尿外科 [3]广东省中医药科学院

出　　处：《现代泌尿生殖肿瘤杂志》2014年第6期356-358,共3页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：广东省自然科学基金项目(S2012010010813);广东省中医院拔尖人才专项(2012-1-4)

摘　　要：目的观察RNA干扰(RNAi)沉默MUC1-C的表达对前列腺癌PC3细胞增殖的影响。方法构建靶向MUC1-C的特异性小干扰RNA(siRNA)表达载体,用脂质体Lipofectamine3000TM转染人前列腺癌PC3细胞,以空白组和转染无关序列的阴性对照组细胞作对照,转染48h后收集样本,采用免疫印迹法检测MUC1-C蛋白的表达,噻唑蓝比色法检测细胞的增殖。结果与阴性对照组比较,转染MUC1-C 48h后的PC3细胞,MUC1-C的表达降低,siRNA-144434、siRNA-144435和siRNA-144436的灰度值分别为1.18、0.72和0.28,差异具有统计学意义(P<0.05)。转染组siRNA-144434、siRNA-144435和siRNA-144436的细胞增殖抑制率分别为(12.55±2.05)%、(15.11±2.70)%和(16.22±3.43)%,siRNA-144436作用较明显,与空白组比较差异有统计学意义(P<0.05)。结论靶向MUC1-C的RNAi可抑制MUC1-C在前列腺癌PC3细胞中的表达,从而延缓PC3细胞的增殖。Objective To explore the effects of the MUC1-C silencing by small interfering RNA （siRNA）on the proliferation of human prostate cancer PC3 cells. Methods The siRNA targeting MUC1-C was transfected into PC3 cells by Lipofectamine 3000TM,the effect was examined by Western blot.The inhibition of cell proliferation was determined by MTT assay. Results Western blot analysis demonstrated that after treatment with MUC1-C-siRNA for 48 h,the expression of MUC1-C was signifi-cantly decreased compared with the negative control group,the gray value of siRNA-144434,siRNA-144435 and siRNA-144436 were respectively 1.18,0.72 and 0.28 （P〈0.05）.The siRNA-144434, siRNA-144435 and siRNA-144436 could significantly inhibit the proliferation of PC3 cells;the inhi-bition rate was （12.55±2.05）%,（15.11±2.70）% and （16.22±3.43）%. Conclusions Silen-cing the MUC1-C gene by RNAi can actively suppress the expression of MUC1-C,decrease the growth of PC3 cells.

关 键 词：前列腺癌 MUC1-C RNA干扰 细胞增殖 

分 类 号：R737.25[医药卫生—肿瘤]