肝组织内性甾体激素受体与慢性乙型肝炎病毒复制及其抗原表达的相关性  

Correlations between the receptor of androgen or estrogen-αfrom liver tissue and hepatitis B with replication or antigen expression in patients with chronic hepatitis B

作　　者：王荣芋[1] 张小楠[2] 张占卿[1] 陆伟[1] 沈芳[3] 冯艳玲[4] 袁正宏[5] 

机构地区：[1]上海市公共卫生临床中心肝炎二科,201508 [2]上海市公共卫生科研中心,201508 [3]上海市公共卫生临床中心检验科,201508 [4]上海市公共卫生临床中心病理科,201508 [5]复旦大学医学分子病毒学重点实验室

出　　处：《肝脏》2014年第10期741-751,共11页Chinese Hepatology

基　　金：国家"863"高技术研究发展计划生物和医药领域重大/重点基金资助项目2006AA02A411

摘　　要：目的探讨肝组织内雄激素受体(AR)和雌激素受体-α(ERα)含量对慢性乙型肝炎(CHB)患者血清HBV DNA载量、HBsAg水平和肝组织内HBsAg、HBcAg表达水平影响。方法 135例经肝活组织检查的CHB患者入选本研究,其中HBeAg阳性80例,HBeAg阴性55例。肝组织内AR mRNA和ERαmRNA采用反转录定量PCR检测。结果HBeAg阳性患者,血清HBV DNA载量与肝组织内AR mRNA和ERαmRNA含量均无显著相关性(r=0.132,P=0.241和r=0.130,P=0.249),血清HBsAg水平与肝组织内AR mRNA和ERαmRNA含量均呈显著正相关(r=0.355,P=0.001和r=0.246,P=0.028);肝组织内HBcAg表达水平与肝组织内AR mRNA和ERαmRNA含量均呈显著正相关(rs=0.438,P=0.000和rs=0.352,P=0.002),肝组织内HBsAg表达水平与肝组织内AR mRNA和ERαmRNA含量无显著相关性(rs=0.112,P=0.333和rs=0.024,P=0.836)。HBeAg阴性患者,血清HBV DNA载量与肝组织内AR mRNA和ERαmRNA含量均无显著相关性(r=-0.256,P=0.061和r=-0.121,P=0.385),血清HBsAg水平与肝组织内AR mRNA和ERαmRNA含量均无显著相关性(r=0.130,P=0.348和r=0.165,P=0.234);肝组织内HBcAg表达水平与肝组织内AR mRNA和ERαmRNA含量均无显著相关性(rs=-0.053,P=0.701和rs=-0.203,P=0.137),肝组织内HBsAg表达水平与肝组织内AR mRNA和ERαmRNA含量无显著相关性(rs=0.159,P=0.247和rs=0.192,P=0.160)。多因素方差分析和有序Logistic回归分析的结果显示,肝组织内AR mRNA为影响HBeAg阳性患者血清HBsAg水平和肝组织内HBcAg表达水平的独立因素。结论肝组织内AR表达水平是影响CHB患者HBV抗原表达的一个独立因素。Objective To inquire into the influences of androgen receptor （AR）and estrogen-α（ERα）in liver tissue in patients with chronic hepatitis B （CHB）on HBV DNA and HBsAg level in serum,and HBsAg and HBcAg expression level in liver tissue. Methods 135 patients with CHB were enrolled in present study,in which 80 patients and 55 patients respectively were HBeAg-positive and HBeAg-negative. AR mRNA and ERαmRNA in liver tissue were assayed by reverse quantitative PCR. Results In HBeAg-positive patients,serum HBV DNA level was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue（r= 0.132,P= 0.241 和r= 0.130,P= 0.249）,and serum HBsAg level was positively correlated significantly with both AR mRNA and ERαmRNA level in liver tissue（r= 0.355,P= 0.001 和r=0.246,P= 0.028）;HBcAg expression level in liver tissue was positively correlated significantly with both AR mRNA and ERαmRNA level in liver tissue（rs= 0.438,P= 0.000 和rs= 0.352,P= 0.002）,,and HBsAg expression level in liver tissue was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue（rs= 0.112,P= 0.333 和rs= 0.024,P= 0.836）. In HBeAg-negative patients,serum HBV DNA level was not correlated significantly with both AR＆amp;nbsp;mRNA and ERαmRNA level in liver tissue （r= -0.256,P= 0.061 and r= -0.121,P= 0.385）,and serum HBsAg level was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue （r= 0.130,P= 0.348 and r= 0.165,P= 0.234）;HBcAg expression level in liver tissue was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue （rs= -0.053,P= 0.701 and rs= -0.203,P= 0.137）,and HBsAg expression level in liver tissue was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue （rs= 0.159,P=0.247 and rs= 0.192,P= 0.160）.The outcomes of multivariate analyses of variance and ordinal logistic regression analyses showed that AR mRNA level in liver tissue was the independent factor influencing HBsA

关 键 词：雄激素受体 雌激素受体-Α 慢性乙型肝炎 病毒复制 抗原表达 ESTROGEN receptor-α 

分 类 号：R512.62[医药卫生—内科学]