NIMA相关蛋白激酶6在肝癌组织的表达及意义  

Expression and significance of NIMA-related kinase 6in hepatocellular carcinoma

作　　者：张标[1] 张海[2] 李相成[3] 

机构地区：[1]苏州高新区人民医院普通外科,江苏省215129 [2]江苏大学附属医院肝胆外科 [3]南京医科大学第一附属医院肝脏外科

出　　处：《江苏医药》2014年第24期2967-2969,F0002,共4页Jiangsu Medical Journal

摘　　要：目的研究NIMA相关蛋白激酶6(NEK6)在肝癌(HCC)组织中的表达及意义。方法采用荧光定量PCR检测168例HCC及癌旁组织中NEK6mRNA表达,分析其与HCC临床病理参数的相关性。通过转染NEK6过表达质粒构建稳定表达NEK6的HCC细胞株,行裸鼠皮下种植,观察NEK6对HCC细胞致瘤能力的影响。结果与癌旁组织比较,HCC癌组织NEK6mRNA阳性表达率高[23.8%(40/168)vs.76.2%(128/168)](P<0.01);NEK6mRNA表达量与肿瘤大小、脉管侵犯及病理分级显著相关(P<0.05)。NEK6过表达可明显促进肝癌细胞株Huh7和HepG2的成瘤能力。结论 HCC组织高表达NEK6,且与HCC恶性程度明显相关,可促进HCC细胞成瘤。Objective To investigate the expression and significance of NIMA-related kinase 6（NEK6）in hepatocellular carcinoma（HCC）.Methods The NEK6 mRNA expression in 168 HCC samples and tumor-adjacent tissues was detected by qRT-PCR.The correlation between NEK6 expression and clinicopathological features of HCC was analyzed.The HCC cell line stably expressing NEK6 was constructed by transfecting recombinant plasmid pcDNA3.1B-NEK6-Flag into HCC cells,which was planted subcutaneously in nude mice for observing the tumorigenesis of NEK6.Results Compared to tumor-adjacent tissues,the expression rate of NEK6 mRNA in HCC was increased [23.8%（40/168）vs.76.2%（128/168）]（P〈0.01）.NEK6 mRNA expression level was significantly correlated with tumor size,vascular invasion and pathological grade（P〈0.05）.The overexpression of NEK6 mRNA enhanced the tumorigenicity of Huh7 and HepG2cells.Conclusion NEK6 is overexpressed in HCC,which is closely correlated with HCC malignancy and may promote the tumorigenesis of HCC cells.

关 键 词：NIMA相关蛋白激酶6 肝癌 

分 类 号：R735[医药卫生—肿瘤]