脊椎动物和寄生虫尿苷磷酸化酶的研究进展  

Research Progress on Uridine Phosphorylase in Vertebrate and Parasites

作　　者：李润花[1] 祝建疆[2] 殷国荣[3] 

机构地区：[1]太原师范学院生物系,太原030031 [2]北京市海淀区妇幼保健院生殖遗传中心,北京100080 [3]山西医科大学医学寄生虫学研究所,太原030001

出　　处：《中国寄生虫学与寄生虫病杂志》2014年第6期465-468,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.81071374);山西省自然科学基金(No.2013011059-4)~~

摘　　要：尿苷磷酸化酶(uridine phosphorylase,UPP)是嘧啶补救合成途径的一种关键性调节酶,可逆地催化尿嘧啶核苷、脱氧尿嘧啶核苷转变为尿嘧啶和核糖-1-磷酸,对维持和调控组织中尿嘧啶核苷的浓度起着至关重要的作用。不同物种的尿苷磷酸化酶虽然在功能上有很多相似之处,但是在理化性质、结构特点、活化位点、与底物结合位点等诸多方面存在差异,因此将尿苷磷酸化酶作为靶点,设计和开发抗菌、抗寄生虫感染药物有着广阔的前景。本文综述不同生物来源的尿苷磷酸化酶蛋白的理化性质及研究进展,为研究刚地弓形虫UPP基因表达蛋白作为抗弓形虫疫苗靶点的可行性提供理论依据。Uridine phosphorylase（UPP） is a key enzyme of pyrimidine salvage pathways， catalyzing the reversible phosphorolysis of ribosides of uracil to nucleobases and ribose 1-phosphate. UPP plays an important role in the regulation of uridine homeostasis. Although UPP from a variety of organisms have many similarities in their functions， there are differences in many other aspects， such as physical and chemical properties， structure characteristics， active sites， and substrate binding sites. Therefore， UPP has broad application prospects in the design and development of antibacterial， antiparasitic drugs. This article summarizes the physico-chemical property and research progress of UPP from a variety of organisms， in order to integrate information of UPP， provide theoretic basis for further study of Toxoplasma gondii UPP protein as a feasible target antigen for toxoplasmosis vaccination.

关 键 词：尿苷磷酸化酶 嘧啶补救途径 刚地弓形虫 疫苗 靶点 

分 类 号：R382.5[医药卫生—医学寄生虫学]