miR101慢病毒表达系统的构建及其在人牙周膜细胞骨向分化中靶向调节PLAP-1基因表达的研究  

Construction of lentiviral miR101 expression system and the role of miR101 in the regulation of PLAP-1 expression during osteogenic differentiation of periodontal ligament cells

作　　者：李长霞[1] 卢昌懿[2] 李春雷[3] 岳静[4,5] 黄欣[4,5] 吴补领[4,5] 

机构地区：[1]广州军区联勤部门诊部,广东广州510080 [2]广州军区广州总医院护理部,广东广州510010 [3]广东省人民医院口腔科,广东广州510080 [4]南方医科大学南方医院 [5]南方医科大学口腔医学院,广东广州510515

出　　处：《牙体牙髓牙周病学杂志》2014年第12期696-700,共5页Chinese Journal of Conservative Dentistry

基　　金：广东省自然科学基金(8451051501000371);广东省科技发展项目(2008B011300018-8);中国教育部研究生奖(20094433120004)

摘　　要：目的:构建miR101的慢病毒表达系统并验证其对牙周膜细胞PLAP-1基因的表达调控。方法:用矿化培养液培养牙周膜细胞,使用p LVX-shRNA1慢病毒表达系统构建获得超表达miR101和抑制表达miR101的慢病毒;用q PCR验证miR101的表达水平,使用Western检测PLAP-1的表达水平。结果:成功获得了miR101的慢病毒表达系统,用于转染矿化诱导液培养的人牙周膜细胞。过表达miR101可抑制PLAP-1的表达,抑制表达miR101可增强PLAP-1的表达。结论:miR101可以调节PLAP-1基因并参与人牙周膜细胞骨向分化中PLAP-1基因表达的调节,miR101可能在人牙周膜细胞骨向分化中发挥调节作用。AIM：To investigate whether miR101 regulates the gene expression of PLAP-1 in human perio-dontal ligament cells （ PDLCs） .METHODS：Overexpression and downexpression systems of miR101 were constructed with pLVX-shRNA1 system.PDLCs were cultured with osteogenesis induction medium and infected by the miR101 overexpression and downexpression systems respectively.Western blot was performed to confirm the effects of these constructs on PLAP-1 gene expression.RESULTS：Constructs of lentiviral-miR101 were obtained.Overexpression of miR101 reduced the expression level of PLAP-1.Downexpression of miR101 increased the expression level of PLAP-1.CONCLUSION：miR101 regulates PLAP-1 expression during osteogenic differentiation of PDLCs, sugges-ting that miR101 may play important roles in the osteogenic differentiation of PDLCs.

关 键 词：PLAP-1 牙周膜细胞 成骨分化 miR101 

分 类 号：R780.2[医药卫生—口腔医学]