细胞牙骨质发育的组织形态学观察  

A histomorphological observation of cellular cementum devolopment in BALB/c mice

作　　者：于西佼[1] 杜言梅[1] 姜欢[1] 杜毅[1] 

机构地区：[1]济南市口腔医院牙体牙髓科,山东济南250001

出　　处：《牙体牙髓牙周病学杂志》2014年第12期701-704,共4页Chinese Journal of Conservative Dentistry

基　　金：济南市科技发展计划(201121043);济南市卫生科技发展计划(2010-32)

摘　　要：目的:通过追踪观察小鼠细胞牙骨质发育过程中的组织形态和细胞活性改变,探讨源于上皮根鞘断裂的上皮性细胞是否参与细胞牙骨质的发育。方法:选取出生后15、19、25 d BALB/c小鼠的下颌第一磨牙根尖1/3区细胞牙骨质,细胞角蛋白14(CK 14)标记追踪上皮根鞘断裂后的上皮性细胞;TUNEL(Td T-mediated-d UTP nick end labeling,TUNEL)法检测细胞牙骨质在发育过程中的细胞凋亡情况;透射电镜观察细胞牙骨质发育的超微结构。结果:细胞凋亡检测结果显示,细胞牙骨质形成过程中可见大量被埋入或正被埋入细胞呈现凋亡阳性表达;免疫组化染色结果显示,其中部分细胞呈CK14阳性表达;透射电镜观察结果显示,细胞牙骨质形成过程中上皮性细胞被其外侧成牙骨质细胞分泌的基质包围。结论:来源上皮根鞘断裂的上皮性细胞可能作为牙骨质细胞参与细胞牙骨质的形成。AIM：To observe the histological and cellular activity changes during cellular cementum develop-ment in BALB/c mice.METHODS：Post-natal 15, 19 and 25d BALB/c mice （8 in each group） were sacrificed and cellular cementum of mandibular first molar was taken.Cytokeratin 14 （ CK14 ） expression in epithelial cells of Hertwig′s epithelial root sheath （ HERS） was examined by PV two-step immunohistological method.The morphology and distribution of epithelial cells and cellular cementum were examined by light and transmission electron microscope （ TEM） .Apoptosis was identified by the terminal deoxy-transferase （ TdT）-mediated dUTP-biotin nick end labeling （ TUNEL） method.RESULTS：During cellular cementogenesis, some cells were incorporated in the cementum and positive CK14 expression can be observed.Following the disintegration of HERS, cementum matrix was rapidly deposi-ted around cementoblasts.Epithelial cells and cementoblasts on the root surface were embedded as cementocytes. CONCLUSION：Epithelial cells from disintegrated HERS may directly participate in cellular cementogenesis.

关 键 词：上皮根鞘( HERS) 细胞牙骨质 细胞角蛋白14 发育 

分 类 号：R780.2[医药卫生—口腔医学]