人脐带间充质干细胞与乳鼠许旺细胞共培养向类神经元样细胞的分化  

Rat's Schwann cells induce neural differentiation of human umbilical cord mesenchymal stem cells

作　　者：祝加学[1] 陶海荣[1] 沈尊理[2] 

机构地区：[1]上海交通大学医学院附属第三人民医院骨科,上海市201999 [2]上海交通大学附属第一人民医院创伤外科中心,上海市200080

出　　处：《中国组织工程研究》2014年第45期7250-7254,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(30872630)~~

摘　　要：背景:间充质干细胞可以通过化学诱导或者共培养的方法分化为类神经细胞,但是对于间充质干细胞是与许旺细胞共培养分化为类神经元还是类许旺细胞,还存在争议。目的:探索大鼠许旺细胞对人脐带间充质干细胞诱导分化作用。方法:将1×109L-1人脐带间充质干细胞通过Transwell隔离小室与1×109L-1SD乳鼠许旺细胞共培养2周,观察脐带间充质干细胞的形态变化,并用免疫荧光法检测其表型变化。结果与结论:共培养2周后,部分间充质干细胞出现类似神经元的形态,且表达nestin,NF-200,β-Ⅲ-tubulin等神经元特异性标记物,不表达许旺细胞特异性表记物S100和胶质细胞特异性标记物MAB1580。提示人脐带间充质干细胞可以与大鼠许旺细胞非接触共培养分化为类神经元。BACKGROUND：Mesenchymal stem cel s can differentiate into nerve cel s by chemical induction or co-culture method, but whether mesenchymal stem cel s co-cultured with Schwann cel s differentiate into neuronal-like cel s or Schwann-like cel s is stil controversial. OBJECTIVE：To explore the inductive role of Schwann cel s derived from rats in the differentiation of human umbilical cord mesenchymal stem cel s. METHODS：Cocultures of human umbilical cord mesenchymal stem cel s （1×109/L） and Schwann cel s （1×109/L） from neonatal rats were performed using transwel culture dishes. After 2 weeks of cocultures, morphology of the cultured human umbilical cord mesenchymal stem cel s was observed, and the phenotypic changes of cel s were also detected with immumocytochemistry techniques. RESULTS AND CONCLUSION：After 2 weeks of cocultures, some differentiated cel s showed neuron-like morphology, and expressed nestin, NF-200 andβ-III-tubulin, but did not express Schwann cel special marker S100 and oligodendrocytes special marker MAB1580. These findings indicate that human umbilical cord mesenchymal stem cel s can transdifferentiate into neuronal-like cel s by cocultures with rat’s Schwann cel s.

关 键 词：干细胞 脐带脐血干细胞 脐带 间充质干细胞 许旺细胞 神经元 细胞培养 共培养 诱导 分化 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]