腮腺肿瘤患者唾液和血液中CEA、CA125含量的检测及其在肿瘤组织中的表达  

CEA and CA125 levels in saliva and serum of patients with parotid tumor and their expression in tumour tissue

作　　者：张凯宇[1] 李钧[2] 刘长营[2] 华琳[3] 

机构地区：[1]首都医科大学口腔医学院口腔颌面外科,北京100050 [2]首都医科大学口腔医学院种植中心,北京100050 [3]首都医科大学统计学教研室

出　　处：《北京口腔医学》2014年第6期331-335,共5页Beijing Journal of Stomatology

基　　金：首都临床特色应用研究(北京市科学技术委员会Z111107058811040)

摘　　要：目的检测腮腺肿瘤患者唾液和血液中CA125、CEA的含量及其在腮腺肿瘤组织中的表达。方法选择腮腺良性肿瘤患者83例,腮腺恶性肿瘤患者18例,对照组为30名健康志愿者。采用化学发光法(CLIA)检测唾液及血液样本中CEA和CA125含量。采用免疫组化法检测腮腺肿瘤组织中CEA和CA125的表达。结果腮腺肿瘤患者混合唾液中CEA和CA125水平明显高于对照组。腮腺肿瘤患者与对照组血液中CEA和CA125水平无显著差异。良、恶性肿瘤患者唾液中CEA和CA125含量无显著差异。CEA及CA125的分布特点为,混合唾液中含量最高,血液中含量最低。相关性分析显示,混合唾液中CEA与CA125呈现明显正相关性(r=0.652-0.913)。在大部分腮腺肿瘤组织中,CEA和CA125均为阳性表达,而且部分病例的肿瘤组织周围正常腺体也可见CEA及CA125的阳性表达。结论唾液中CEA和CA125水平的变化可以反映腮腺肿瘤的存在,但无法区分腮腺肿瘤的良恶性。血液中CEA和CA125不能反映腮腺肿瘤的存在。腮腺肿瘤患者的混合唾液样本中CEA与CA125呈现同步变化的趋势。唾液中CEA与CA125可能主要来源于涎腺腺体及肿瘤组织,而不是来自血液。Objective To determine the levels of CEA and CA125 in saliva and serum of patients with parotid tumor and their expression. Methods A total of 101 patients with unilateral parotid tumor and 30 healthy subjects were included in this study. Concentrations of CEA and CA125 were measured with chemiluminescence immunoassay （ CLIA） , and their expression in tumor tissues from 18 patients were examined by immunohistochemistry. Results Levels of CEA and CA125 in whole saliva were significantly higher in patients than in the controls, but the levels of CEA and CA125 in serum showed no significant difference among control and patients with benign and malignant tumors. There was no significant difference in saliva level between benign and malignant tumors. The highest levels of CEA and CA125 were found in saliva, while the lowest in serum. Analysis showed that positive correlation was found between CEA and CA125 in whole saliva. CEA and CA125 were expressed in most of parotid tumor tissue and in the acinus adjacent to tumor tissue of some cases. Conclusion The elevated levels of CEA and CA125 in saliva could reflect the occurrence of parotid tumor, but the levels of CEA and CA125 in serum showed no significant difference between patients and the control. It is possible that CEA and CA125 in saliva may originate from parotid tumor and gland instead of serum.

关 键 词：唾液 血液 CEA CA125 腮腺肿瘤 

分 类 号：R739.8[医药卫生—肿瘤]