基于核磁代谢组学的驴皮与其伪品的鉴别研究  

Identification of donkey-hide and its counterfeits based on 1^H-NMR metabolomics

作　　者：田俊生[1] 那丽丹 向欢[3] 陈建丽[1] 马晓晴[2] 秦雪梅[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]山西大学体育学院,山西太原030006

出　　处：《中草药》2015年第2期255-261,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81102833);山西省基础研究项目(2012021031-2);山西大学本科生科研训练项目(2014012105)

摘　　要：目的使用核磁共振(NMR)代谢组学技术对驴Equus asinus皮及其伪品(马E.caballus皮、骡E.ferus×asinus皮)的水提物进行鉴别研究,为阿胶的鉴别和质量控制研究提供依据。方法从去毛后的驴皮及其伪品中提取水溶性成分,然后进行核磁共振氢谱(1H-NMR)分析,对所得的核磁数据进行水溶性成分归属指认,并进行多元统计分析。结果在驴皮及其伪品1H-NMR图谱中指认出了42种水溶性成分。与驴皮比较,马皮中含较多醋酸、亮氨酸、缬氨酸、异亮氨酸,含较少乳酸、丝氨酸、焦谷氨酸、肌酸,而骡皮中含较多乳酸、肌酸、丙氨酸、胆碱,含较少甘油、醋酸,并且都具有显著性差异(P<0.05)。结论初步建立了基于核磁代谢组学技术对阿胶原料驴皮与其伪品的鉴别研究方法,为阿胶原料驴皮的鉴别和质量控制研究提供实验依据。Objective The aqueous extracts of donkey-hide and its counterfeits（horse-hide and mule-hide） were identified using 1H-NMR metabolomics to provide the basis for the identification and quality control of donkey-hide gelatin.Methods The water-soluble components of the hairless donkey-hide and its counterfeits were extracted and the 1^H-NMR technique was applied to analyzing the components.Moreover,the NMR data were analyzed and the component belongings were recognized using relevant software for the multivariate statistical analysis.Results Forty-two water-soluble components were identified in donkey-hide by 1H-NMR.There were more acetate,leucine,valine,isoleucine and less lactate,serine,pyroglutamate,and creatine in the horse-hide than in the donkey-hide;While there were more acetate,creatine,alanine,choline and less glycerol and acetate in mule-hide than in donkey-hide.And they were all in a significant difference（P 〈0.05）.Conclusion The identification method of the raw material of Colla Corii Asini,namely,donkey-hide and its counterfeits based on 1H-NMR metabolomics was established for the first time,which provides the experimental references for the donkey-hide identification and quality control study.

关 键 词：驴皮 阿胶 代谢组学 核磁共振 伪品鉴别 

分 类 号：R284.1[医药卫生—中药学]