不同碳源对重组毕赤酵母表达嗜热β-半乳糖苷酶的影响  

Effect of Different Carbon Sources on Thermostabilityβ- Galactosidase Expression by Recombinant Pichia Pastoris

作　　者：李洪波[1,2] 郭祥前 陈静[1] 龙腾[1] 张子政[1] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]怀化学院民族药用植物资源研究与利用湖南重点实验室,湖南怀化418008

出　　处：《怀化学院学报》2014年第11期17-20,共4页Journal of Huaihua University

基　　金：湖南省高校科技创新团队支持计划项目(2010212);湖南省重点学科建设项目(2011042)

摘　　要：为分析碳源对嗜热β-半乳糖苷酶在组成型重组毕赤酵母中的表达水平,葡萄糖、果糖、丙三醇、乳糖、蔗糖和麦芽糖分别用于配制培养基的碳源.当以单糖和甘油为碳源时,酵母的生长和重组嗜热β-半乳糖苷酶的分泌水平要优于双糖;以果糖为碳源时,重组酵母分泌嗜热β-半乳糖苷酶的水平最高,其次为葡萄糖和丙三醇,蔗糖要高于麦芽糖,乳糖最低.To analyze the thermostabiliuy of recombinant β- Galactosidase secretory expression level in the constitutive recombinant Pichia pastoris, glucose, fructose- glycerol, lactose, sucrose and maltose were used as carbon sources respectively. The results showed that monosaccharide and glycerol were better than disaccharide in cell growth and secretory expression of recombinant β- Galactosidase. The secretory expression level of thermostability recombinant β- Galactosidase in fructose was best than the other 5 carbon resources, glucose second and followed by glycerol, sucrose better than maltose,lactose was the lowest.

关 键 词：分泌表达 碳源 重组 毕赤酵母 Β-半乳糖苷酶 

分 类 号：Q786[生物学—分子生物学]