肾透明细胞癌差异表达miRNAs的筛选  

Identification of differentially expressed miRNAs in renal clear cell carcinoma

作　　者：肖耀军[1] 陈壮飞[2] 郑少斌[2] 王海坤[1] 黄谷[1] 李彬[1] 

机构地区：[1]武警广东总队医院泌尿外科,广东广州510507 [2]南方医科大学附属南方医院泌尿外科,广东广州510505

出　　处：《广州医科大学学报》2014年第4期13-18,共6页Academic Journal of Guangzhou Medical University

摘　　要：目的:筛选肾透明细胞癌组织差异表达的miRNAs,并分析其与可能的靶基因作用调控关系。方法:收集8例新鲜的肾透明细胞癌及其癌旁正常肾组织,抽提总RNA,并行Hy3荧光标记,将标记的小RNA与包含1891条探针的miRNA芯片进行杂交,再扫描及数据分析处理。miRNA芯片结果的可靠性采用RT-PCR方法验证。结果:肾透明细胞癌组织与癌旁正常肾组织比较,存在较多明显差异表达的miRNAs,其中上调miRNAs 106个、下调miRNAs 184个,其中111个miRNAs为16.0版miRNA芯片新增的;显著上调的miRNAs 3个、下调的miRNAs 38个。结论:肾透明细胞癌组织的miRNA表达谱有较强特异性,其与可能的靶基因调控关系复杂,其中miR-155、miR-141、miR-200c及let-7家族等与肾透明细胞癌的发病密切相关。Objective：To investigate the differentially expressed miRNAs and analyze their regulatory relations with possible target genes.Methods： Total RNA was extracted from 8 fresh samples of renal clear cell carcinoma and adjacent nontumorous tissues. Small miRNAs were isolated from total RNA, labeled with Hy3 and hybridized with the miRNA array, which included 1891 probes, for screening and data processing and analyzing. Real-time quantitative PCR （ RT-PCR） was applied to validate the reliability of miRNA array results. Results：Compared to normal adjacent tissues, miRNAs were differentially expressed in cc-RCC, including 106 up-regulated and 184 down-regulated miRNAs, with 111 added miRNAs of 16. 0 version miRNA array. 3 miRNAs were significantly up-regulated and 38 miRNAs were significantly down-regulated. Conclusion： The miRNA expression profile of human cc-RCC has strong specificity, which has complex relationship with possible target genes. Such as miR-155, miR-141, miR-200c and let-7 family, etc, may be closely related to the pathogenesis and development of cc-RCC.

关 键 词：MIRNA 肾透明细胞癌 MIRNA芯片 实时定量RT-PCR 

分 类 号：R737.11[医药卫生—肿瘤]