白血病细胞抗原负载脐血DCs体外诱导抗白血病特异性CTLs应答研究  

Specific CTL response of anti-leukemia induced by leukemia antigen loading with cord blood DCs in vitro

作　　者：刘芯[1] 谭丽[1] 谭获[1] 

机构地区：[1]广州医科大学附属第一医院肿瘤血液中心,广东广州510230

出　　处：《广州医科大学学报》2014年第4期26-29,共4页Academic Journal of Guangzhou Medical University

摘　　要：目的:探讨体外诱导脐血产生特异性细胞毒淋巴细胞的可行性。方法:通过体外联合细胞因子体外诱导10份脐血单个核细胞分化为树突状细胞(dendritic cells,DCs),同时让DCs细胞负载U937冻融抗原;使成熟DCs刺激同源的脐血T淋巴细胞生成细胞毒性T细胞(cytotoxic T lymphocytes,CTLs)从而进行杀伤效应实验。MTT法测定杀伤活性。结果:10份脐血标本均可培养出形态典型、功能成熟的DCs。经DCs诱导效应细胞DC-CTLs,在不同效靶比对U937细胞系、K562细胞株和对照组中,杀伤率以U937组最高(P<0.05)。结论:特定抗原负载的脐血DCs细胞有特异性的杀伤效应。Objective：To investigate the feasibility of inducing specific cytotoxic T lymphocytes by cord blood in vitro. Methods：Mononuclear cells from 10 induced samples of cord blood were differentiated into dendritic cells （ DCs） by cytokines in vitro, and DCs cells were loading with freeze-thaw antigen of U937 at the same time. Homologous cord blood T lymphocytes activated by mature cells generated cytotoxic T lymphocytes （ CLTs） for the experiment of lethal effect. The killing activity was assayed with MTT method. Results： Mature DCs with typical morphology and function were cultivated from 10 samples of cord blood. As effector cell DC-CTLs were induced through DCs, the target ratio was compared amongU937 cell line group, K562 cell line group and the control group. It showed that the highest killing rate was in U937 group （P〈0.05）. Conclusion： DCs cells of cord blood loaded with specific antigen have specific killing effect.

关 键 词：细胞毒性T淋巴细胞 脐血 树突状细胞 免疫治疗 

分 类 号：R733.7[医药卫生—肿瘤]