不同浓度CGF提取液对成骨细胞影响的实验研究  

The effects of concentrate growth factors extract(CGF e) on osteoblasts

作　　者：姜波[1] 董凯[2] 柳忠豪[2] 

机构地区：[1]山东大学齐鲁医院高新区医院,250101 [2]滨州医学院附属烟台市口腔医院

出　　处：《中国口腔种植学杂志》2014年第4期160-163,共4页Chinese Journal of Oral Implantology

摘　　要：目的:探讨不同浓度浓缩生长因子提取液(CGF,Concentrate Growth Factors extract,CGF e)对成骨细胞增殖、分化的影响。方法:实验组分别使用含有10%CGF e、20%CGF e浓度的α-MEM培养液培养细胞,对照组则使用不含CGF e的α-MEM培养液。甲基噻唑基四唑(MTT)法测定1天,3天,5天的细胞增殖数;碱性磷酸酶(ALP)活性检测3天,5天,7天的细胞分化情况;荧光实时定量PCR测定核心结合蛋白因子2(Runt-related transcription factor-2,RUNX2)和成骨细胞特异性转录因子(Osterix,OSX)基因分别在3天,7天的表达。结果:MTT示,随培养时间延长,细胞数量逐渐增加,在各时间点,20%CGF e浓度组的吸光度值最高,和对照组相比,10%及20%CGF e浓度组的吸光度值均显著提高,差异有统计学意义(P<0.05);ALP活性,在每一时间点,20%CGF e浓度组的吸光度值最高,10%及20%CGF e浓度组的吸光度值均显著高于对照组(P<0.05);荧光实时定量PCR,随着培养时间延长,各组细胞的基因表达量逐渐增加,将对照组的基因表达水平定义为1,进行组内标准化和对照组相比较,两组实验组基因表达量均显著增高(P<0.05),其中,20%CGFe浓度组细胞的基因表达量最高。结论:CGF e能有效地促进成骨细胞的增殖、分化,20%浓度的CGF e较10%浓度的CGF e更能促进成骨细胞的增殖、分化。Objective： To evaluate the effect of different concentrations Concentrate Growth Factors extract （CGF e） on proliferation and differentiation of osteoblasts. Methods： Trials are divided into two experi-mental groups and the control group. Experimental groups use the α-MEM containing 10% CGF e and 20% CGF e respectively, while group （D） just use the α-MEM. MTT assay to detect the number of the osteoblasts at 1d、3d、5d; the activity of alkaline phosphatase （ALP） to detect the differentiation of os-teoblast at 3d、5d、7d; the level of osteogenetic biomarkers RUNX2 and OSX at 3d、7d were quantified by real-time PCR. Results：MTT assay indicated that at 1d、3d、5d,both the 10%CGF e and 20%CGF e have a significant increase of absorbance, the most higher increase was 20% CGF e group （P＆lt;0.05）;ALP activity showed that at 3d、5d、7d, the highest absorbance was 20% CGF e group, the absorbance of 10% and 20% CGF e group were significant higher than control group （P＆lt;0.05）. Realtime PCR： As the standardization in the group, the gene expression level of control group were defined as 1,the RUNX2 and OSX gene expression in experimental group are larger than control group, the highest gene expression was 20% CGF e group. Conclusions： Our work confirmed that CGF e is useful in stimulat-ing the proliferation and differentiation of osteoblasts. The 20% CGF e showed higher effect on os-teoblast than 10%CGF e.

关 键 词：浓缩生长因子 骨结合 种植体 成骨细胞 

分 类 号：R783.6[医药卫生—口腔医学]