提高广枣药材质量标准研究  

Improvement of quality standard for Choerospondiatis Fructus

作　　者：龙凯花 刘峰[2,3] 王春柳[4] 杨东花 李彤晖[4] 辛永洁[4] 李晔[4] 

机构地区：[1]陕西中医学院,咸阳712046 [2]西安交通大学药学院天然药物与工程中心,西安710061 [3]陕西步长制药有限公司,西安710075 [4]陕西省中医药研究院,西安710003

出　　处：《西北药学杂志》2015年第1期14-16,共3页Northwest Pharmaceutical Journal

基　　金：国家"十二五重大新药创制"科技重大专项(编号:2011ZX09401-308-6)

摘　　要：目的提高广枣药材的质量控制标准。方法采用薄层色谱法（TLC）对广枣进行定性鉴别,高效液相色谱法（HPLC）同时测定广枣中没食子酸与原儿茶酸的含量。结果 TLC对没食子酸与原儿茶酸分离度均良好;没食子酸在3.53~70.60μg·mL^-1范围内线性关系良好,平均加样回收率为103.8%,RSD为1.5%（n=6）;原儿茶酸在6.24~124.8μg·mL^-1范围内线性关系良好,平均加样回收率为101.4%,RSD为1.1%（n=6）。结论该定性方法专属性强,定量方法简便、快捷、准确,可作为广枣药材的质量控制方法。Objective To improve the quality standard of Choerospondiatis Fructus .Methods Thin layer chromatography （TLC） was used to identify Choerospondiatis Fructus ,and high performance liquid chromatography （HPLC） was used to determine the gallic acid and protocatechuic acid content in the Choerospondiatis Fructus .Results TLC showed excellent performance for both gallic acid and protocatechuic acid ;gallic acid in the range of 3 .53‐70 .60μg · mL^-1 showed good linear relationship .The average recovery was 103 .8% ,and RSD was 1 .5% （n=6）;protocatechuic acid in the range of 6 .24‐124 .8μg · mL^-1 showed good line‐ar relationship .The average recovery was 101 .4% ,and RSD was 1 .1% （n=6） .Conclusion The qualitative method is specific . The quantitative method is simple ,fast and accurate ,and can be used for the quality control of Choerospondiatis Fructus .

关 键 词：没食子酸 原儿茶酸 广枣 质量标准 TLC HPLC 

分 类 号：R282[医药卫生—中药学]