猪链球菌广东株的分离鉴定及药敏试验  

Isolation,Identification and Drug Sensitivity Test of Isolates of Streptococcus suis in Guangdong Province

作　　者：彭凌[1] 杨旭夫[1] 朱必凤[1] 刘博婷[1] 

机构地区：[1]韶关学院粤北生猪生产及疫病防控协同创新发展中心、英东生命科学学院、中国农业科学院哈尔滨兽医研究所一韶关学院动物疫病诊断中心联合实验室,广东韶关512005

出　　处：《动物医学进展》2015年第2期130-133,共4页Progress In Veterinary Medicine

摘　　要：对来自广东韶关某猪场的疑似猪链球菌病病料进行分离鉴定,共分离到3株链球菌,对这3株链球菌进行了形态学观察、生化试验、16 S rRNA基因序列分析、猪链球菌2型的毒力基因检测及药敏试验。结果表明,其形态、染色、生化特性均符合猪链球茵的特点,测序后Blast同源性分析,证实3株菌株均为猪链球菌,用猪链球菌2型Cps2J引物进行PCR扩增,获得预期的459bp大小的目的片段。药敏试验结果表明,该菌对3种常用抗生素表现出敏感,对21种常用抗生素表现出耐药。Isolation and identification of Streptococcus suis in Shaoguan Guangdong Province were conducted,and 3 strains were obtained. The morphology, biochemistry test,sequence analysis of 16 S rRNA gene, detection of virulence genes of Streptococcus suis serotypes 2 and drug sensitivity test were done on them. The results were as follows：their morphology, dyeing characteristics, biochemical characteristics were in coincidence with characteristics of Streptococcus suis. The sequences of 16 S rRNA of 3 strains were obtained successfully. The sequences were compared with related sequences through online Blast in NCBI, showing the 3 strains were Streptococcus suis. Thruogh this PCR ,which is based on the amplification of the cps2J gene coding for the capsule of Streptococcus suis serotypes 2, a fragmentof 459 bp corresponding to a part of the cps gene coding for the capsule was only obtained with. Streptococcus suis serotypes 2 strains as expectancy. Drug susceptibility test results showed that the isolates were drug resistant to 21 kinds of common antibiotic.

关 键 词：猪链球菌 16 S RRNA 荚膜多糖 药敏试验 

分 类 号：S852.611[农业科学—基础兽医学] S858.28[农业科学—兽医学]