共轭亚油酸与运动对青春期肥胖大鼠肝脏及脂肪组织视黄醇结合蛋白4的影响  

Effects of conjugated linoleic acid and exercise on RBP4 of liver and adipose tissues in adolescent obese rats

作　　者：崔建飞[1] 柏友萍[1] 李萌[1] 许晓东[1] 戴园园[1] 张晶[1] 

机构地区：[1]安徽师范大学体育学院,芜湖241003

出　　处：《卫生研究》2015年第1期8-14,共7页Journal of Hygiene Research

基　　金：安徽省高校自然科学基金重点项目(No.KJ2013A138)

摘　　要：目的探讨共轭亚油酸（CLA）与运动对青春期肥胖大鼠肝脏及脂肪组织视黄醇结合蛋白4（RBP4）的影响。方法选取喂饲普通饲料的青春期SD大鼠8只为空白对照组（C）,高脂组建模7周选取32只肥胖鼠随机分为4组,每组8只,分别为安静对照组（OC）、安静补CLA组（OCC）、运动组（OM）、运动结合CLA组（OMC）。动物跑台跑速为21~25 m/min,60 min/次;CLA灌胃1.6 g/kg,1次/日,5次/周,连续8周。实验结束后麻醉动物,采集血液及组织,测血糖、计算胰岛素敏感性,检测内脏脂肪组织RBP4 mRNA表达、肝脏组织RBP4蛋白表达及血浆RBP4浓度。结果干预后OM、OMC组体重、体重增长幅度和体脂百分比、血糖、内脏脂肪组织RBP4mRNA表达、肝脏组织RBP4蛋白表达、血浆RBP4浓度低于OC、OCC组（P〈0.01）。OM、OMC组胰岛素敏感性高于OC、OCC组（P〈0.01）。OMC组体重、体重增长幅度、胰岛素敏感性高于OM组,体脂百分比、血糖、RBP4表达低于OM组,但差异无统计学意义。结论单纯运动与运动结合CLA显著降低青春期肥胖大鼠的体重、体重增长幅度、体脂百分比、血糖,提高胰岛素敏感性及降低内脏脂肪组织RBP4的mRNA表达、肝脏组织RBP4蛋白表达、血浆RBP4水平,运动与运动结合CLA效果明显优于单纯补CLA。Objective To exploer effects of conjugated linoleic acid and exercise on RBP4 of liver and adipose tissues in adolescent obese rats. Methods 8 adolescent SD rats were selected as blank control（C）with normal diet. Obese rats were modeled with high fat feeding for 7 weeks. 32 obese rats were selected and randomly divided into 4 groups： control group （ OC）, CLA group （ OCC）, exercise group （ OM）, exercise combined with CLA group （OMC）. Exercise treatment were performed on animal treadmill with running speed of 21 -25 m/min, 60 rain/every time. For CLA treatment, each rat was fed 1.6 g/kg, one time/ day, 5 times/week, for 8 weeks. Animals were anesthesia treated after experiment, blood and body tissues were collected. The content of blood glucose was measured, the insulin sensitivity was calculated, testing the expression of RBP4 mRNA in visceral adipose tissue, expression of RBP4 protein in liver cells and plasma RBP4 concentration. Result （1） Weight, weight growth rate and body fat percentage of OM, OMC groups were lower than OC, OCC groups （P 〈 0.01 ）. （2） Blood glucose content of OM, OMC groups were significantly lower than OC, OCC groups（P 〈0.01 ） ,while insulin sensitivity of OM, OMC groups was higher than OC, OCC groups （P 〈 0.01 ）. （3） Expression of RBP4 mRNA of OM, OMC groups in visceral adipose tissue was lower than OC, OCC groups （P 〈 0.01 ）. Expression of RBP4 protein of OM, OMC groups in liver cells was significantly lower than OC, OCC groups. RBP4 concentration in plasma of OM, OMC groups was lower than OC, OCC groups （P 〈 0.01 ）. （4） Weight, weight growth rate, insulin sensitivity of OMC group were higher than OM group, but the percentage of body fat, blood glucose content and the expression of RBP4 were lower than OM group, there were no significant differences between them. Conclusion Simple exercise and exercise combined with CLA conld significantly reduced adolescent obese rats weight, weight growth rate, percentage of body fat, bl

关 键 词：共轭亚油酸 运动 青春期肥胖 脂肪 肝脏 视黄醇结合蛋白4 

分 类 号：R589.2[医药卫生—内分泌] Q591[医药卫生—内科学]